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ENZYMES THAT ARE L-GLUTAMINASES EXHIBIT ANTITUMOR ACTIVITY.

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4741
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4741
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Engenics Inc
3760 Haven Avenue Menlo Park, CA 94025
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: ENZYMES THAT ARE L-GLUTAMINASES EXHIBIT ANTITUMOR ACTIVITY.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

ENZYMES THAT ARE L-GLUTAMINASES EXHIBIT ANTITUMOR ACTIVITY. THUS, WORK TO DEVELOP THESE ENZYMES INTO THERAPEUTIC AGENTS IS IN PROGRESS. PRESENT LIMITATIONS INCLUDE THE ENZYMES' SUBSTRATE AFFINITY, SIZE, IMMUNOGENICITY, AND, IN SOME CASES, DUAL FUNCTION AS BOTH L-GLUTAMINASE AND L- ASPARAGINASE. THE OBJECTIVE OF THIS PROJECT IS TO OBTAIN A SINGLE-FUNCTION L-GLUTAMINASE THAT IS SMALLER IN SIZE. PHASE I INCLUDES BOTH THE CLONING AND EXPRESSION OF THE GENEENCODING PSEUDOMONAS 7A L-GLUTAMINASE-L-ASPARAGINASE, AN ENZYME WITH VERY HIGH AFFINITY FOR L-GLUTAMINE. NOT ONLY WILL PHASE I WORK PROVIDE A LARGER AND CHEAPER SOURCE OF ENZYME FOR CLINICAL TRIALS, BUT IT WILL ALSO ALLOW THE PURSUIT OF THE SECOND PHASE OF THE PROJECT. PHASE II WILL OBTAIN, THROUGH OLIGONUCLEOTIDE AND DELETION MUTAGENESIS, ANENZYME THAT IS EXCLUSIVELY AN L-GLUTAMINASE AND THAT IS SUFFICIENTLY SMALL TO ALLOW FOR IMPROVED PENETRABILITY OF TUMORS LOCATED IN THE EXTRAVASCULAR SPACE. THE ANTITUMOR ACTIVITY OF THE RESULTANT GENETICALLY-ENGINEERED ENZYMES WILL BE TESTED BY INJECTING THEM INTO TUMOR-BEARING MICE. DEPENDING UPON THE SIMILARITIES BETWEEN PROKARYOTIC AND EUKARYOTIC L-GLUTAMINASES, THE CLONED PSEUDOMONAS 7A GENE MIGHT ALSO SERVE AS A PROBE TO ISOLATE A HUMAN, NONIMMUNO- GENIC, L-GLUTAMINASE.

Principal Investigator:

Carol a talkington
PRINCIPAL INVESTIGATOR
4153248600

Business Contact:

Small Business Information at Submission:

Engenics Inc
3760 Haven Avenue Menlo Park, CA 94025

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No