A method to develop a model system for nonradioactive dna hybridization/detection for identification of bacteria will be developed. dna for ampicillin resistance will be labeled with biotinylated deoxyuridine triphosphate by nick translation and used as probe. colony hybridization procedures for the… More

Strategies are proposed for developing sensitive, rapid and easy to use methods for nonradioactive detection of dna: dna hybrids using biotin labeled dna probes. the approaches are directed toward incorporating a greatly increased number of biotin molecules into dna molecules associated with the… More

Tests directed for use in clinical diagnostic laboratories.the paramyxoviruses, respiratory syncytial virus (rsv), and parainfluenza virus are the chief respiratory pathogens of human infancy. a specific, quick, easy, and economical diagnostic test would greatly aid the clinician in diagnosis and… More

Human papilloma virus (hpv) infections are a common form of sexually transmitted disease. infections with two strains of hpv, hpv-16 and hpv-18, have been correlated with cervical carcinoma. dna from these viruses has been detected in tumor tissue from patients with this malignancy. in phase i of… More

Nonradioactive dna-based detection systems offer an attractive new approach to the clinical diagnosis of a variety of viral and bacterial pathogens. current methods for labeling dna by enzymatic procedures are time consuming,labor intensive, and not well suited for large-scale production. this… More

The aim of this study is to develop a nonradioactive, homogeneous hybridization assay for identifying bacterial and viral pathogens in clinical specimens. hybridization will be monitored using a novel time-resolved fluorescence (trf) instrument to detect fluorescence resulting from energy transfer… More

The aim of this project is to develop a rapid, highly sensitive assay for simultaneously detecting the in situ hybridization of multiple human papilloma virus (hpv) type-specific dna probes to the hpv dna in a single specimen. in phase i, a model system consisting of two well-characterized cervical… More

The goal of this program is to use antisense, transgenic technologies to develop a murine hepatitis virus (mhv)-resistant strain of mice. during phase i, research will: (1) test the antiviral efficacy, in tissue culture, ofantisense oligonucleotides complementary to specific regionsof the mhv… More

The goal of this research is to use specific antisense sequences to inhibit, in vitro, acute infection of lymphoid cells by hiv. phase ieefforts will include: (1) examinationof the process of dna replication and integration during hiv infection of lymphoid cell lines using in situ hybridization and… More