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CYTOMEGALOVIRUS DETECTION USING NONRADIOACTIVE DNA PROBES IN SITU

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
5012
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
5012
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Ert
9653 Featherwood Lane Spring Valley, OH 45370
View profile »
Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: CYTOMEGALOVIRUS DETECTION USING NONRADIOACTIVE DNA PROBES IN SITU
Agency: HHS
Contract: N/A
Award Amount: $214,000.00
 

Abstract:

CYTOMEGALOVIRUS (CMV), A MEMBER OF THE HERPES VIRUS GROUP, IS PRESENT IN THE BLOOD OF A LARGE PORTION OF THE HUMAN POPULATION. ADMINISTRATION OF THIS BLOOD TO HIGH-RISK PATIENTS MAY RESULT IN DEATH. ENZO PROPOSES THE DEVELOPMENTOF A QUICK, SPECIFIC, EASY AND ECONOMICAL DIAGNOSTIC TEST, BASED ON NONRADIOACTIVE DNA-DNA HYBRIDIZATION, TO DIRECTLY SCREEN HUMAN BLOOD FOR CMV. PHASE I DEVELOPMENT WILL BE PERFORMED USING CMV-INFECTED HUMAN FIBROBLAST MONOLAYERS MIXED WITH HUMAN BLOOD OR BUFFY COAT LEUKOCYTES AS A MODEL SYSTEM. DETECTION OF VIRAL DNA WILL BE BASED ON IN SITU HYBRIDIZATION OF NONRADIOACTIVE BIOTINYLATED CMV-SPECIFIC DNA PROBES. VISUALIZATION WILL INVOLVE DETECTION OF BIOTIN BASED ON ENZYMATIC OR FLUORESCENT METHODS. THIS MODEL WILL ALLOW FOR OPTIMIZATION OF SAMPLE TREATMENT AND HYBRIDIZATION-DETECTION CONDITIONS IN ORDER TO ATTAIN A RELIABLE AND STRAIGHTFORWARD DIAGNOSTIC ASSAY THAT CAN BE PERFORMED IN THE TYPICAL CLINICAL OR BLOOD LABORATORY. PHASE II WILL INVOLVE TESTING THIS DIRECT ASSAY ON HUMAN BLOOD SAMPLES. THE RESULTS WILL BE COMPARED WITH THOSE OBTAINED FROM STANDARD CELL CULTURE ASSAYS.

Principal Investigator:

John A Todd
Principal Investigator
2127413838

Business Contact:

Small Business Information at Submission:

Enzon Inc.
300 Corporate Ct Middlesex Business Ctr South Plainfield, NJ 08854

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No