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CHARACTERIZATION OF A POTENTIAL NEW ANTIMICROBIAL AGENT

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13617
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13617
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epicentre Technologies Corporation
1202 Ann Street Madison, WI 53713 0241
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: CHARACTERIZATION OF A POTENTIAL NEW ANTIMICROBIAL AGENT
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

RECENTLY, RESEARCHERS FOUND THAT THE BACTERIAL PHYTOTOXIN TAGETITOXIN IS A POTENT INHIBITOR OF ESCHERICHIA COLI RNA POLYMERASE IN VITRO. THIS COMMON MECHANISM OF ACTION WITH KNOWN ANTIBIOTICS SUCH AS RIFAMPICIN AND STREPTOLYDIGINSUGGESTS THAT TAGETITOXIN OR ITS DERIVATIVES HAVE POTENTIAL AS NEW ANTIMICROBIALS. STRUCTURAL INFORMATION OBTAINED TO DATE INDICATES THAT TAGETITOXIN IS MUCH DIFFERENT THAN THE KNOWN ANTIBIOTICS THAT ARE RNA POLYMERASE INHIBITORS. THE GOALS OF PHASE I RESEARCH ARE: (1) TO INVESTIGATE MEDIA AND FERMENTATION PARAMETERS THAT WILL ALLOW EFFICIENT AND CONSISTENT GROWTH OF THE TOXIN-PRODUCING BACTERIUM, ALONG WITH PRODUCTION OF REPRODUCIBLY HIGH LEVELS OF TAGETITOXIN; (2) TO SCALE UP AND IMPROVE PURIFICATION METHODS; AND (3) TO DETERMINE THE EXACT STRUCTURE OF TAGETITOXIN BY USING INSTRUMENTAL, CHEMICAL, AND ENZYMATIC METHODS. THE STRUCTURAL KNOWLEDGE OBTAINED IN PHASE I WILL BE USED IN PHASE II TO SYNTHESIZE AND EVALUATE NEW TAGETITOXIN ANALOGS FOR BACTERIAL PERMEABILITY, RNA POLYMERASE INHIBITORY ACTIVITY, ANTIMICROBIAL ACTIVITY, AND STRUCTURE/ACTIVITY RELATIONSHIPS, WITH THE ULTIMATE AIM OF DEVELOPING TAGETITOXIN OR ITS DERIVATIVES INTO A NEW FAMILY OF ANTIMICROBIAL PRODUCTS FOR HUMAN AND/OR VETERINARY MEDICINE.

Principal Investigator:

Jerome Jendrisak
6082518474

Business Contact:

Small Business Information at Submission:

Epicentre Technologies Corp
1202 Ann St Madison, WI 53713

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No