Rapid Detection Nano-Sensors for Biological Warfare Agents in Buildings and HVAC Systems

In the Phase I program, EPIR Technologies successfully developed a technology for real-time, simultaneous detection of several types of biological agents using a novel FRET-based detection technique. The development of multiplexed detection capabilities with extremely low false positives in this Phase I program allowed EPIR to establish improved standards in biosensing. Innovative surface modification protocols for bioconjugation of quencher labeled bioagents enabled the shelf life of these complexes to be extended greatly. In the proposed Phase II program, EPIR will develop the Phase I "proof of concept" to prototyping, while continuously working towards the advancement of the FRET-based detection to go beyond the current state of the art biosensors for HVAC systems. To sustain a commercially viable biosensor program, EPIR has already initiated the development of on-site facilities to lower production costs and expand process quality control. EPIR also proposes to investigate innovative design and engineering processes to develop low cost, miniature biosensors. Finally, EPIR proposes several innovative "smart decontamination" techniques that will be integrated to the biosensors to not only detect but also provide large scale, rapid decontamination of the air inside buildings and HVAC systems.