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DETECTION OF AIDS VIRUS (HTLV-III) BY MONOCLONAL ANTIBODIES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4978
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
4978
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epitope Inc.
15425-e Sw Koll Parkway Beaverton, OR 97006
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1988
Title: DETECTION OF AIDS VIRUS (HTLV-III) BY MONOCLONAL ANTIBODIES
Agency: HHS
Contract: N/A
Award Amount: $406,000.00
 

Abstract:

THE OBJECTIVE OF THIS PROPOSAL IS TO USE MOUSE MONOCLONAL ANTIBODIES TO HTLV-III TO EVALUATE THE FEASIBILITY OF DETECTING HTLV ANTIGEN EXPRESSION ON T LYMPHOCYTES IN CLINICAL SPECIMENS FROM PATIENTS WITH LYPHADENOPATHY SYNDROME AND OVERT AIDS, AND IN INDIVIDUALS IN HIGH RISK GROUPS. THIS WILL BE DONE BY: (1) PRODUCING AN ARRAY OF MOUSE MONOCLONALS TO HTLV-III ANTIGENS BY IMMUNIZING WITH SELECTED ANTIGENS TO EXTENSIVE IMMUNIZATION PROTOCOLS; (2) SELECTING HYBRID CULTURES REACTIVE TO HTLV-III BY ELISA SCREENING AND WESTERN BLOTTING AND TO CLONE AND CHARACTERIZEHYBRIDS WHICH EXPRESS SELECTIVE REACTIVITY PATTERNS TO HTLV-III; (3) TO EVALUATE SELECTED MONOCLONAL ANTIBODIES FOR USE IN DETECTING HTLV-III ANTIGEN EXPRESSION ON HUMAN T LYMPHO- CYTES BY FLOW CYTOMETRY; AND (4) TO USE SELECTED MONOCLONAL ANTIBODIES IN A RETROSPECTIVE TRIAL TO COMPARE (A) ELISA REACTIVITY, (B) WESTERN BLOTTING, (C) T LYMPHOCYTE H/S RATIO, AND D HTLV ANTIGEN EXPRESSION BY FLOW CYTOMETRY ON A BANK OF SELECTED SAMPLES.

Principal Investigator:

Denis R Burger
Principal Investigator
5036416115

Business Contact:

Small Business Information at Submission:

Epitope Inc.
15425-e Southwest Koll Parkway Beaverton, OR 97006

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No