USA flag logo/image

An Official Website of the United States Government

HIGH RESOLUTION MUTAGENESIS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
8026
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
8026
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genesys Research, Inc.
2300 ENGLERT DR Durham, NC 27514
View profile »
Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: HIGH RESOLUTION MUTAGENESIS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE FEASIBILITY OF PROBING HIGH RESOLUTION MUTAGENESIS AT THE THYMIDINE KINASE (TK) LOCUS IN L5178Y MOUSE LYMPHOMA CELLS WILL BE EXAMINED (A) TO MORE THOROUGHLY EVALUATE THE HYPOTHESIS THAT SMALL (PHI) COLONIES IN THE BIMODAL DISTRIBUTION OF L5178Y CELLS MUTANTS ARE THE RESULT OF MORE EXTENSIVE DAMAGE TO THE REGION OF THE TK GENES (CHROMOSOME 1LB) AND THAT LARGE (LAMBDA) COLONIES RESULT FROM POINT MUTATIONS TO THE TK GENE, AND (B) TO CHARACTERIZE PHI AND LAMBDA MUTANTS ARISING IN L5178Y MOUSE STRAINS 3.7.2C, LY-S1, AND LY-R83 GROWN IN FOLIC ACID-DEFICIENT MEDIUM THEN EXPOSED TO ETHYLMETHANE SULFONATE. THE PHENOTYPE OF MUTANT CULTURES AT THE TK AND GALACTOKINASE (GK) LOCI WILL BE CHARACTERIZED; HIGH RESOLUTION BANDED CHROMOSOMES (NUMBER 11) WILL BE ANALYZED, AND IN SITU HYBRIDIZATION OF RADIOACTIVE PROBES OF TK AND GK CDNA TO THE HIGH-RESOLUTION BANDED CHROMOSOMES WILL BE INITIATED. IN PHASE II THESE APPROACHES WILL BE CONTINUED AND THE MUTANTS WILL BE CHARACTERIZED AT THE MOLECULAR LEVEL.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Genesys Research Inc
2520 Wyandotte St Mountain View, CA 94043

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No