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STABILIZATION OF ENZYMES BY ATTACHMENT TO SOLUBLE POLYMERS

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
1800
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
1800
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genetics Institute Inc.
87 Cambridge Park Drive Cambridge, MA 02140
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: STABILIZATION OF ENZYMES BY ATTACHMENT TO SOLUBLE POLYMERS
Agency: NSF
Contract: N/A
Award Amount: $200,000.00
 

Abstract:

HYPOTHESES ABOUT THE STABILIZATION OF ENZYMES BY COVALENT ATTACHMENT TO SOLUBLE POLYMERS ARE TO BE TESTED IN DETAIL TO ISOLATE THE EFFECT OF MULTI-POINT ATTACHMENT FROM OTHER FACTORS KNOWN TO AFFECT ENZYME STABILITY INCLUDING CHEMICAL MODIFICATION, REDUCED PROTEOLYSIS, DIFFUSIONAL EFFECTS, AND SUBUNIT DISSOCIATION. TWO MODEL ENZYME SYSTEMS HAVE BEEN SELECTED BASED ON CRITERIA WHICH WILL ALLOW MEASUREMENT OF THE EFFECT OF MULTI-POINT ATTACHMENT ALONE. EACH ENZYME WILLBE COVALENTLY ATTACHED THROUGH ITS LYSINE RESIDUES TO TWO DIFFERENT ACTIVATED, SOLUBLE POLYMERS. STABILITY TO HEAT, PH CHANGE, AND DENATURATION BY UREA WILL BE STUDIED AS A FUNCTION OF THE NUMBER OF POINTS OF ATTACHMENT TO THE SOLUBLE POLYMER.

Principal Investigator:

J. David Rozzell Jr.
Senior Scientist
6178761170

Business Contact:

Small Business Information at Submission:

Genetics Institute, Inc.
87 Cambridge Park Dr Cambridge, MA 02140

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No