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IMMUNE COMPLEXES (IC) PLAY AN IMPORTANT ROLE IN THE PATHOGENESIS OF CERTAIN…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
494
Program Year/Program:
1983 / SBIR
Agency Tracking Number:
494
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genetics Systems Corpon
3005 First Ave. Seattle, WA 98121
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1983
Title: IMMUNE COMPLEXES (IC) PLAY AN IMPORTANT ROLE IN THE PATHOGENESIS OF CERTAIN HUMAN DISEASES, SUCH AS SERUM SICKNESS, GLOMERULONEPHITIDES, AND LUPUS ERYTHEMATOSUS, AND ARE ALSO ASSOCIATED WITH RHEUMATOID ARTHRITIS AND A VARIETY OF VASCULITIDES.
Agency: HHS
Contract: N/A
Award Amount: $72,851.00
 

Abstract:

IMMUNE COMPLEXES (IC) PLAY AN IMPORTANT ROLE IN THE PATHOGENESIS OF CERTAIN HUMAN DISEASES, SUCH AS SERUM SICKNESS, GLOMERULONEPHITIDES, AND LUPUS ERYTHEMATOSUS, AND ARE ALSO ASSOCIATED WITH RHEUMATOID ARTHRITIS AND A VARIETY OF VASCULITIDES. PRESENT TESTS FOR THE DETECTION OF IC RELYON PHYSIOCHEMICAL METHODS OR BIOLOGICALLY BASED ASSAYS WHICHSEPARATE IC FROM FREE ANTIBODY (AB). THE VARIOUS TESTS USEDDO NOT CORRELATE WELL WITH EACH OTHER, HAVE PROBLEMS IN THEIR SENSITIVITY AND/OR SPECIFICITY, AND DO NOT DISTINGUISHIC OF DIFFERENT IMMUNOGLOBULIN ISOTYPES OR SUBCLASSES. THERE IS A REAL NEED FOR A MORE SPECIFIC, SENSITIVE, AND EASY IC ASSAY FOR USE IN DIAGNOSIS, DETERMINING PROGNOSIS, AND MONITORING THERAPEUTIC INTERVENTION. WE PROPOSE TO DEVELOP MONOCLONAL ANTIBODIES (M.AB) SPECIFIC FOR IC WHICH DO NOT REACT WITH EITHER FREE ANTIGEN (AB) OR AB. THESE M.AB. MAY ENABLE IC OF DEFINED ISOTYPES TO BE DETECTED IN A HOMOGENEOUS ASSAY WITHOUT SEPARATION OF BOUND FROM UNBOUND AB. TO DEMONSTRATE THE FEASIBILITY OF THIS APPROACH, WE HAVE SELECTED A MURINE SYSTEM WITH WELL-DEFINED ANTIGENS (HAPTENS) AND WELL-DEFINED ANTIGEN OR HAPTEN-SPECIFIC ANTIBODIES OF DIFFERENT ISOTYPES. BOTH MONOVALENT HAPTEN (DINITROPHENOL, DNP) AND POLYVALENT DNA ANTIGENS WILL BE USED. FIRST, WE WILL DEVELOP M.AB WHICH REACT WITH HAPTEN- CARRIER-ANTIHAPTEN OR DNA-ANTI DNA IMMUNE COMPLEXES, BUT NOT WITH AG OR AB ALONE. SECOND, WE WILL DETERMINE IF ANY OF THE IC-SPECIFIC M.AB ARE SPECIFIC FOR CERTAIN ISOTYPES. THIRD, WE WILL DEMONSTRATE THAT THE EPITOPES RECOGNIZED BY THESE M.AB ARE DUE TO CONFORMATIONAL CHANGES IN THE BOUND ABAND NOT SIMPLY DUE TO AGGREGATION OR DIMER FORMATION. FINALLY, WE WILL BEGIN TO TEST THESE M.AB IN ASSAYS FOR IC IN SERA FROM MICE WITH AUTOIMMUNE DISEASE.

Principal Investigator:

Edward a. clark ph.d.
PRINCIPAL INVESTIGATOR
2066244300

Business Contact:

Small Business Information at Submission:

Genetics Systems Corpon
3005 First Ave. Seattle, WA 98121

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No