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SYNTHESIS OF A SCA PROTEIN WITH ENZYMATIC ACTIVITY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13884
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13884
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genex Corpon
16020 Industrial Dr Gaithersburg, MD 20877
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: SYNTHESIS OF A SCA PROTEIN WITH ENZYMATIC ACTIVITY
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

A SINGLE-CHAIN ANTIBODY (SCA) PROTEIN WITH CATALYTIC ACTIVITY WILL BE PREPARED. THE SCA PROTEIN WILL BE DESIGNEDFROM THE VARIABLE REGION DEQUENCES OF ONE OF TWO ANTIBODIES FOUND TO HAVE LIPASE ACTIVITY, EITHER 2H6 OR 21H3. A GENE TO SYNTHESIZE THIS PROTEIN WILL BE CONSTRUCTED, AND THE PROTEIN WILL BE EXPRESSED IN ESCHERICHIA COLI. THE PROTEIN WILL BE RENATURED, PURIFIED, AND ASSAYED FOR ITS ENZYMATIC ACTIVITY. THIS SCA PROTEIN WILL BE ENGINEERED TO HAVE INCREASED STABILITY AND INCREASED CATALYTIC ACTIVITY DURING PHASE II OF THE PROJECT. THE SCA PROTEIN WITH CATALYTIC ACTIVITY PROVIDES THE MEANS TO ACCOMPLISH GENETIC CHANGES AND DETERMINE THE EFFECT ON CATALYTIC ACTIVITY AND STABILITY.

Principal Investigator:

Leslie S Johnson
3012580552

Business Contact:

Small Business Information at Submission:

Genex Corp
16020 Industrial Dr Gaithersburg, MD 20877

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No