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ENGINEERING OF NEW BINDING SPECIFICITIES OF PROTEIN A

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13620
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13620
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genex Corpon
16020 Industrial Dr Gaithersburg, MD 20877
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: ENGINEERING OF NEW BINDING SPECIFICITIES OF PROTEIN A
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE OBJECTIVE OF THIS RESEARCH IS TO USE PROTEIN ENGINEERING TO GENERATE STAPHYLOCOCCAL PROTEIN A VARIANTS THAT POSSESS STRONG BINDING AFFINITY FOR IMMUNOGLOBULIN SPECIES THAT ARE NOT SIGNIFICANTLY BOUND BY NATURAL PROTEIN A. THE EXPERIMENTAL PROGRAM WILL INVOLVE A SEQUENTIAL SERIES OF EXPERIMENTS OF INCREASING LEVELS OF DIFFICULTY ENCOMPASSING STRUCTURAL DESIGN, VARIANT CONSTRUCTION, AND TESTING OF IMMUNOGLOBULIN BINDING. BASED ON THE EXISTING PROTEIN A/IMMUNOGLOBULIN FC COCRYSTAL STRUCTURE, COMPUTER MODELING AND GENETIC ENGINEERING TECHNOLOGIES WILL BE USED TO DESIGN AND PRODUCE PROTEIN A VARIANTS WITH HIGH ENGINEERED AFFINITY FOR (1) HUMAN IGG3, (2) MOUSE IGG1, AND (3) HUMAN IGM FOR THE CLINICAL DIAGNOSTICS AND THERAPEUTICS MARKET. IF SUCCESSFUL, THIS MODIFICATION OF THE BINDING SPECIFICITY OF A PROTEIN-PROTEIN COMPLEX WILL BE A MILESTONE IN THE DEVELOPMENT OF PROTEIN ENGINEERING AND WILL ALSO SERVE AS A PREFACE TO THE ENGINEERING OF PROTEIN A VARIANTS THAT BIND OTHER IMMUNOGLOBULIN CLASSES, E.G., IGE, IGD, AND IGA. THE ENGINEERED AFFINITY PROTEIN FOR HUMAN IGM WILL HAVE UTILITY AS A DIAGNOSTIC REAGENT FOR ANTIGEN-SPECIFIC IGM AND AS A THERAPEUTIC IN EXTRACORPOREAL BLOOD FILTRATION FOR DISEASES INCLUDING CANCER AND AIDS.

Principal Investigator:

David R Filpula
3012580552

Business Contact:

Small Business Information at Submission:

Genex Corp
16020 Industrial Dr Gaithersburg, MD 20877

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No