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Method for Analyzing Non-poly(A) RNA Transcripts

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
89188
Program Year/Program:
2008 / SBIR
Agency Tracking Number:
GM083526
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
GENHUNTER CORPORATION
624 GRASSMERE PARK DR, STE 17 NASHVILLE, TN 37211-3671
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2008
Title: Method for Analyzing Non-poly(A) RNA Transcripts
Agency: HHS
Contract: 1R43GM083526-01A1
Award Amount: $149,800.00
 

Abstract:

DESCRIPTION (provided by applicant): Much efforts in gene expression analysis in the past have been focused mainly on the messenger RNAs (mRNAs), thanks to the availability of Differential Display (DD), SAGE and DNA microarray technologies, which all targ et the poly(A) tails present in most eukaryotic mRNAs. The recent discovery of a large microRNA population begged the question of whether there exists additional yet to be discovered RNAs species in a eukaryotic cell besides mRNA, rRNA and tRNA. However, i n contrast to the analysis of mRNA expression, analogous methods for an accurate, comprehensive detection and analysis of any nonpolyadenylated RNA have been lacking. Here we describe a systematic approach for the detection and identification of any non-po lyadenylated RNAs in a eukaryotic cell. The method involves first in vitro enzymatic addition of a poly(A) tail to all non-poly(A) RNAs in a cell followed by fluorescent Differential Display (FDD) comparison of cDNA patterns before and after poly(A) additi on. With the proof of principle established for the method, two well defined specific aims are formulated in this Phase I STTR application to further optimize and streamline the method for a more accurate and comprehensive screen for nonpolyadenylated RNA species expression in any eukaryotic cell. Specific Aim 1: Systematic Analysis of Non-poly(A) RNA Expression in Eukaryotic Cells by Differential Display a: Optimization of poly(A) tailing reaction of NPA-DD b: Poly(A) tailing of total RNA following the dep letion of poly(A) RNA and comparison of NPA-DD with tiling arrays, c: NPA-DD bypassing ribosomal RNA detection. Specific Aim 2: Comprehensive Test Screens for Non-poly(A) RNA Expression in Mammalian Cells.

Principal Investigator:

Business Contact:


jwalden@genhunter.com
Small Business Information at Submission:

GENHUNTER CORPORATION
GENHUNTER CORPORATION 624 GRASSMERE PARK DR, STE 17 NASHVILLE, TN 37211

EIN/Tax ID: 104317381
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No