THE DEVELOPMENT OF CELL LINES EXPRESSING RAT CYTOCHROME P450CDNAS

Award Information

Agency: Department of Health and Human Services

Branch: N/A

Contract: N/A

Agency Tracking Number: 13854

Amount: $343,945.00

Phase: Phase II

Program: SBIR

Solicitation Topic Code: N/A

Solicitation Number: N/A

Timeline

Solicitation Year: N/A

Award Year: 1991

Award Start Date (Proposal Award Date): N/A

Award End Date (Contract End Date): N/A

Small Business Information

GENTEST CORPORATION

6 Henshaw St

Woburn, MA 01801

United States

DUNS: N/A

HUBZone Owned: No

Woman Owned: No

Socially and Economically Disadvantaged: No

Principal Investigator

Name: Charles L Crespi
Phone: (617) 935-5115

Business Contact

Phone: () -

Research Institution

N/A

Abstract

THIS PROJECT WILL DEVELOP HUMAN CELL LINES EXPRESSING RAT CYTOCHROME P450 CDNAS. TWO RAT CYTOCHROME P450 CDNAS, ENCODING P450IIB1 AND P450IID1, WILL BE TRANSFECTED INTO THEAHH-1 TK+/- LINE OF HUMAN LYMPHOBLASTOID CELLS. VECTORS CONTAINING THE EPSTEIN-BARR VIRUS ORIGIN OF REPLICATION WILL BE USED IN THIS TRANSFECTION. RECOMBINANT CELL POPULATIONS WILL BE ISOLATED, AND SPECIFIC CYTOCHROME P450 CATALYZED REACTIONS WILL BE MEASURED IN RECOMBINANT AND CONTROL CELL POPULATIONS. THE RECOMBINANT CELL LINES WILL ALSO BE USED IN GENE MUTATION ASSAYS. PROMUTAGENS KNOWN TO BE ACTIVATED BY THE SPECIFIC P450 FORMS WILL BE TESTED. THE EVENTUAL GOAL OF THIS PROGRAM IS TO DEVELOP A PANEL OF CELL LINES EXPRESSING RAT CYTOCHROME P450S. THIS PANEL CAN BE USED TO ANALYZE RAT FOREIGN COMPOUND METABOLISM WITH THE ABILITY TO RELATE METABOLITE FORMATION WITH THE INDUCTION OF GENOTOXICITY IN THE SAME CELL LINE. ADDITIONALAPPLICATIONS INCLUDE ANALYSIS OF STRAIN- OR SEX-SPECIFIC CYTOCHROME P450S AND ALSO COMPARISON TO HUMAN P450S IN THE SAME CELL SYSTEM.

* Information listed above is at the time of submission. *

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THE DEVELOPMENT OF CELL LINES EXPRESSING RAT CYTOCHROME P450CDNAS