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THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4711
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4711
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genzyme Corp.
75 Kneeland St Boston, MA 02111
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS. IT IS ANTICIPATED THAT BY EMPLOYING A COMBINATION OF CHROMATOGRAPHIC TECHNIQUES ON AFFINITY, HIGH-PERFORMANCE ANION EXCHANGE, AND/OR HIGH PERFORMANCE GEL FILTRATION MATRICES, HOMOGENEOUS ACIDIC A-D-MANNOSIDASE WILLBE OBTAINED. THIS WILL PERMIT THE GENERATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES DURING PHASE II. FURTHERMORE, THEPURIFIED ENZYME AND ANTISERUM WILL PROVIDE A MEANS FOR CLONING THE GENE FOR ACIDIC A-D-MANNOSIDASE. THE ABSENCE OFTHIS ENZYME IN THE LYSOSOMAL STORAGE DISORDER, MANNOSIDOSIS,WHICH OCCURS IN BOTH MAN AND CATTLE, MAKES IT AN IDEAL CANDIDATE FOR STUDYING THE FEASIBILITY OF GENE REPLACEMENT THERAPY.

Principal Investigator:

Shirish hirani
PRINCIPAL INVESTIGATOR
6174511923

Business Contact:

Small Business Information at Submission:

Genzyme Corp
75 Kneeland Street Boston, MA 02111

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No