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A NOVEL APPROACH FOR ENHANCING IN-SITU BIOREMEDIATION OF TRICHLOROETHYLENE

Award Information

Agency:
Environmental Protection Agency
Branch:
N/A
Award ID:
18759
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
18759
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Geo-microbial Technologies, In
PO Box 132 Ochelata, OK 74051
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: A NOVEL APPROACH FOR ENHANCING IN-SITU BIOREMEDIATION OF TRICHLOROETHYLENE
Agency: EPA
Contract: N/A
Award Amount: $49,866.00
 

Abstract:

TRICHLOROETHYLENE (TCE) IS A WIDESPREAD POLLUTANT AND IS REPORTED TO BE THE MOST FREQUENTLY ENCOUNTERED CONTAMINANT AT HAZARDOUS WASTE SITES. ALL PREVIOUS ATTEMPTS AT IN SITU BIOREMEDIATION HAVE USED A PROCESS WHERE METHANTROPHIC BACTERIA WERE STIMULATED BY THE SUBSURFACE DELIVERY OF AIR AND METHANE RESULTING IN A "FORTUITOUS" COMETABOLISM OF TCE. THIS PROCESS AT ONE TIME APPEARED PROMISING FOR THE IN SITU BIOREMEDIATION OF TCE, BUT ONLY LIMITED SUCCESS HAS BEEN ACHIEVED AND NO FIELD SITE HAS BEEN BIOREMEDIATED TO EPA ACCEPTABLE LEVELS. THIS LIMITED SUCCESS IS PRIMARILY DUE TO PHYSICAL PROBLEMS ASSOCIATED WITH DELIVERING SUFFICIENT LEVELS OF METHANE AND OXYGEN WHICH ARE WERY INSOLUBLE. THEREFORE, AN INNOVATIVE APPROACH TO IN SITU BIOREMEDIATION WOULD BE TO FACILITATE TCE COMETABOLISM BY USING HIGHLY SOLUBLE COMPOUNDS. NITRIFYING BACTERIAL ELABORATE AN ENZYME (AMMONIA OXYGENASE) THAT IS A BROAD SPECIFICITY OXYGENASE SIMILAR TO THE BROAD SPECIFICITY ENZYME ELABORATED BY BACTERIA STIMULATED BY METHANE AND AIR. ALSO SOME HETEROTROPHIC BACERIA CAN ELABORATE AMMONIA OXYGENASE WHILE SIMULTANEOUSLY RESPIRING WITH NITRATE THUS DECREASING THEIR OXYGEN REQUIREMENTS. THE OBJECTIVE OF THIS PROPOSAL IS TO STIMULATE TCE COMETABOLISM BY STIMULATING HETEROTROPHIC BACTERIA THAT CAN SIMULTANEOUSLY NITRIFY AND DENTRIFY USING THE WATER SOLUBLE COMPOUNDS, AMMONIA, NITRATE, AND ACETATE.

Principal Investigator:

Dr Kenneth A Sandbeck
Principal Investigator
9185352281

Business Contact:

Small Business Information at Submission:

Geomicrobial Technologies Inc
Po Box 132 Ochelata, OK 74051

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No