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Bioluminescent Detection of Chemical Agent Contamination

Award Information

Department of Defense
Defense Advanced Research Projects Agency
Award ID:
Program Year/Program:
2006 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
Guild Associates, Inc.
5750 Shier Rings Rd P.O. Box 8013 Dublin, OH 43016-2013
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 2
Fiscal Year: 2006
Title: Bioluminescent Detection of Chemical Agent Contamination
Agency / Branch: DOD / DARPA
Contract: W31P4Q-06-C-0474
Award Amount: $374,947.00


Chemical weapons such as organophosphate (OP) nerve agents pose a deadly risk to military and civilian personnel since they are easily dispersed, they persist in the environment, and are extremely toxic. In addition, OP pesticide contamination causes 1 to 3 million unintentional poisonings annually worldwide. Consequently, there is an urgent need for natural biological systems that can detect and biodegrade these toxic compounds. This proposal will generate a Saccharomyces cerevisiae biocatalyst/biosensor that can detect and biodegrade OP agents such as VX, which is the most lethal and persistent OP. S. cerevisiae, which is a robust non-pathogenic microorganism that is resistant to environmental extremes, will be used as a self-contained biocatalyst/biosensor. Importantly, the yeast biosensor will also be able to detect the subsequent VX biodegraded products and will thereby provide a means for monitoring the decontamination process. The goal is to generate a biocatalyst/biosensor which: (i) will require minimal consumables; (ii) will be cheap to produce in bulk quantities; (iii) will emit distinct spectral properties following VX detection and biodegradation, and (iv) will be visually assessed using a simple hand held illumination device. The generation of a system that can detect and biodegrade OPs, and follow the decontamination process, is novel.

Principal Investigator:

Dave Schofield
PI/Research Scientist

Business Contact:

Marcie Willem
Project Administrator
Small Business Information at Submission:

5750 Shier Rings Rd Dublin, OH 43016

EIN/Tax ID: 310998683
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No