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Identifying targets for diagnosing oral candidiasis

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
76134
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
DE017033
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Guild Associates, Inc.
5750 Shier Rings Rd P.O. Box 8013 Dublin, OH 43016-2013
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2005
Title: Identifying targets for diagnosing oral candidiasis
Agency: HHS
Contract: 1R43DE017033-01
Award Amount: $115,939.00
 

Abstract:

DESCRIPTION (provided by applicant): Candida species are normal commensals of the oral cavity, mucosal surfaces, and gastrointestinal tract in healthy subjects but under physiological and immunological changes in the host, can cause a variety of maladies ranging from mucosal infections of the tissues and skin to life threatening systemic disease. Candida albicans is the major fungal pathogen responsible for oral candidiasis which commonly occurs in immunosuppressed patients, diabetics, patients taking antibiotics, infants, smokers and HIV-positive patients. Diagnosis of oral candidiasis is complicated, however, by the fact that current diagnostic methodologies are time consuming, may not differentiate between the different Candida species, and most importantly, do not distinguish between asymptomatic Candida carriers and patients with oral candidiasis; this can result in misdiagnosis leading to increased morbidity, increased expense and the over-prescription of antifungals. The goals of this Phase I application are to identify C. albicans gene biomarkers which are expressed solely during oral infection. Using a unique gnotobiotic mouse model of oral candidiasis, we will use suppressive subtracted hybridization and global transcription profiling to identify and compare C. albicans genes expressed during oral tissue infection to genes expressed during alimentary tract colonization. Potential disease biomarkers will be verified using candidate approaches and prioritized. The ultimate goal of this work is to develop the disease biomarkers discovered in Phase I, into a quick, sensitive and specific PCR diagnostic assay to identify patients infected with Candida species and to differentiate Candida colonizing the healthy host from patients with oral candidiasis. Since Candida species are the most important fungal pathogen of the oral cavity, the rapid and accurate diagnosis of oral candidiasis using novel disease biomarkers will significantly improve oral health.

Principal Investigator:

David A. Schofield
8435730095
DSCHOFIELD@GUILDASSOCIATES.COM

Business Contact:


6147988215
marcie@guildassociates.com
Small Business Information at Submission:

Guild Associates, Inc.
Guild Associates, Inc. 5750 Shier-Rings Rd Dublin, OH 43016

EIN/Tax ID: 310998683
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No