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SELECTIVE CAPTURE AND QUANTITATION OF SERINE PROTEASES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11309
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
11309
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HAEMATOLOGIC TECHNOLOGIES, INC.
HAEMATOLOGIC TECHNOLOGIES, INC. 57 RIVER RD ESSEX JUNCTION, VT 05452
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1990
Title: SELECTIVE CAPTURE AND QUANTITATION OF SERINE PROTEASES
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

THROMBOSIS AND THROMBOLYSIS ARE MAINTAINED IN A DELICATE BALANCE BY A SERIES OF ENZYMATIC REACTIONS. THE COMPONENT ENZYMES ARE PRODUCED AS NEEDED FROM ZYMOGENS, INACTIVE FORMS THAT CIRCULATE IN THE BLOOD AT RELATIVELY HIGH CONCENTRATIONS. IDENTIFYING AND EVALUATING PATIENTS AT RISKFOR CLINICAL THROMBOTIC DISORDERS WILL REQUIRE ACCURATE MEASUREMENT OF PLASMA LEVELS OF THESE ACTIVE PRO- AND ANTICOAGULANT FACTORS. SPECIFIC PROBES HAVE BEEN USED TO BIND TO AND IRREVERSIBLY INHIBIT SERINE PROTEASES, SUCH AS THOSE ASSOCIATED WITH THROMBOSIS AND THROMBOLYSIS. HAEMATOLOGIC TECHNOLOGIES, INC., HAS SYNTHESIZED A FORM OF ONE SUCH PROBE CONTAINING A SECONDARY RECOGNITION SITE THAT CAN BE USED TO CAPTURE THE ENZYME ONTO A SOLID PHASE. SUCH A PROBE HAS BEEN USED, ALONG WITH AN ENZYME-CONJUGATED MONOCLONAL ANTIBODY SPECIFICFOR TISSUE-TYPE PLASMINOGEN ACTIVATOR (TPA), TO PREPARE A PROTOTYPE ASSAY FOR THIS SERINE PROTEASE. THIS PHASE I PROJECT HAS THE FOLLOWING SPECIFIC AIMS: (1) TO OPTIMIZE THE CONDITIONS FOR PROBE PRODUCTION, (2) TO DETERMINE OPTIMAL CONDITIONS FOR USE OF THE PROBE IN WHOLE BLOOD AND PLASMA, AND (3) TO FURTHER DEVELOP THE TPA ASSAY FOR USE WITH CLINICAL SPECIMENS.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Haematologic Technologies Inc.
57 River Rd Box 1021 Essex Junction, VT 05452

EIN/Tax ID: 030312741
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No