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DEVELOPMENT OF SEX REVERSAL TECHNOLOGY FOR CULTURED MARINE SHRIMP AND…

Award Information

Agency:
Department of Agriculture
Branch:
N/A
Award ID:
14116
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
14116
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Hawaii Aquaculture Co Inc
1103 9th Ave Suite 206 Honolulu, HI 96816
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: DEVELOPMENT OF SEX REVERSAL TECHNOLOGY FOR CULTURED MARINE SHRIMP AND FRESHWATER PRAWN BROODSTOCK
Agency: USDA
Contract: N/A
Award Amount: $49,251.00
 

Abstract:

FRESHWATER PRAWNS (MACROBRACHIUM ROSENBERGII) AND SOME MARINE SHRIMP (PENAEUS SP) HAVE SEXUAL DIMORPHISM WHICH MANDATES THAT SEX OF THESE SPECIES BE MANAGED SEPARATELY. MONOSEX AND SKEWED SEX RATIO PROGENY GROUPS CAN BE GENERATEDFORM MATING SEX REVERSED PARENTAL BROODSTOCK. THE TECHNOLOGY TO DO THIS COULD BE BASED UPON SEX REVERSAL USINGTHE ANDROGENIC HORMONE (AH) FROM THE ANDROGENIC GLAND (AG) -AN ORGAN RESPONSIBLE FOR MASCULINIZATION. WHEN AH OR AG IS DELIVERED TO SEXUALLY UNDIFFERENTIATED FEMALES THEY DEVELOP INTO FUNCTIONING MALES. THE WORK PROPOSED HERE FOCUSES ON THE INITIAL DEVELOPMENT OF A DELIVERY SYSTEM TO SEX REVERSE PRAWNS AND SHRIMP USING PRAWN AG AND LYOPHILIZED CELL-FREE EXTRACT (LCE). CROSS SPECIES REACTIVITY IS TESTED USING PRAWN AG IMPLANTS INTO MARINE SHRIMP. ENTERIC COAT LCE WILLALSO BE DELIVERED TO THE JUVENILE AND OVIGEROUS PRAWNS AND SHRIMP BY INJECTION TESTING DOSAGE LEVELS OF 5, 10, AND 20 PRAWN ANDROGENIC GLAND UNITS (PAGUS). EVIDENCE OF MASCULINIZATION WILL BE JUDGED BY THE APPEARANCE OF MALE SECONDARY SEX APPENDAGE CHARACTERISTICS AND INITIATION OF ACUTE DEGENERATION OF OOCYTES. RESULTS ANTICIPATED ARE THAT THE CELL-FREE EXTRACT CONTAINING THE PRAWN ANDROGENIC GLAND HORMONE WILL CAUSE AN ACUTE POSITIVE REACTION IN THE FEMALE RECIPIENT - OVARIES WILL BEGIN TO DEGENERATE AND MALE SECONDARY CHARACTERISTICS WILL APPEAR. MARINE SHRIMP WILL RESPOND TO GLANDULAR IMPLANTS IN A SIMILAR FASHION. EVENTUAL COMMERCIAL APPLICATION INCLUDES THE ABILITY TO REAR ONE SEX OR THE OTHER WITH A 20-30% INCREASE IN PRODUCTION.

Principal Investigator:

Dr Spencer R Malecha
0

Business Contact:

Small Business Information at Submission:

Hawaii Aquaculture Co Inc
1103 9th Ave Suite 206 Honolulu, HI 96816

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No