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HETEROLOGOUS PROTEIN EXPRESSION IN NEUROSPORA

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
10670
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
10670
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HAWAII BIOTECH, INC.
99-193 AIEA HEIGHTS DRIVE, SUITE 200 AIEA, HI -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: HETEROLOGOUS PROTEIN EXPRESSION IN NEUROSPORA
Agency: NSF
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE PROPOSED RESEARCH WILL INVESTIGATE THE ABILITY OF NEUROSPORA CRASSA TO EXPRESS AND SECRETE BIOLOGICALLY-ACTIVEMAMMALIAN PROTEINS. THE ABILITY TO GENETICALLY ENGINEER BACTERIA AND YEASTS FOR THESE TASKS IS WELL DOCUMENTED. HOWEVER, RECOMBINANT PRODUCTS FROM THESE ORGANISMS OFTEN REQUIRE COSTLY DOWNSTREAM PURIFICATION AND PROCESSING. THE PHYSIOLOGY OF THE FILAMENTOUS FUNGI INDICATE THAT THEY ARE EXCELLENT MICROBES TO EFFICIENTLY EXPRESS, MODIFY, AND SECRETE HETEROLOGOUS PROTEINS. SOME INDUSTRIAL FUNGAL STRAINS SECRETE ENDOGENOUS ENZYMES IN MILLIGRAM PER LITER QUANTITIES. THUS, THE INEXPENSIVELY CULTURED FILAMENTOUS FUNGI PROVIDE THE OPPORTUNITY TO SIGNIFICANTLY REDUCE THE COST OF PRODUCING PHARMACOLOGICALLY ACTIVE PROTEINS BY MICROORGANISMS. THE PROPOSED RESEARCH WILL PLACE CDNAS ENCODING RAT RELAXIN AND BOVINE CHYMOSIN UNDER THE REGULATION OF THE N.CRASSA BETA-TUBULIN GENE PROMOTER AND TERMINATOR, AND TEST THE ABILITY OF NEUROSPORA TO EXPRESS THE RECOMBINANT GENES AND TO SECRETE THE HETEROLOGOUS PROTEINS USING THE HETEROLOGOUS PROTEINS' OWN SECRETION SIGNAL PEPTIDES.

Principal Investigator:

John Marshall Ivy
Principal Investigator
8084865333

Business Contact:

Small Business Information at Submission:

Hawaii Biotechnology Group Inc
99-193 Aiea Heights Dr Aiea, HI 96701

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No