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ORGANIC PHASE IMMUNOASSASY FOR CHLORINATED DIBENZODIOXINS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19218
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
19218
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HAWAII BIOTECH, INC.
99-193 AIEA HEIGHTS DRIVE, SUITE 200 AIEA, HI -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: ORGANIC PHASE IMMUNOASSASY FOR CHLORINATED DIBENZODIOXINS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

HIGH AFFINITY ANTIGEN-ANTIBODY BINDING IS ESSENTIALLY ANHYDROUS HYDROPHOBIC ORGANIC SOLVENTS HAS BEEN DEMONSTRATED IN OUR LABORATORY. USING AN ESTRADIOL-ANTIESTRADIOL SYSTEM, A PROTOTYPE ORGANIC PHASE RADIOIMMUNOASSAY (OPRIA) HAS BEEN DEVELOPED WHICH DETECTS 200 PG/ML OR ESTRADIOL IN A HEXANE MEDIUM CONTAINING LESS THAT 0.1% (W/W) WATER. THE RESEARCH WILL RESULT IN THE DEVELOPMENT OF AN OPRIA FOR POLYCHLORINATED DIBENZO-P-DIOXINS (PCDDS). THE ASSAY WILL EMPLOY THE MONOCLONAL ANTIBODIES DEVELOPED AT LAWRENCE LIVERMORE NATIONAL LABORATORY AN THE CROSS-REACTIVE ANTIGEN, [(3)H]1-N-ACETAMIDO-3,7,8-TRICHLORODIBENZO-P-DIOXIN([(3)H]ATRICDD). ATRICDD IS NOT A REGULATED HAZARDOUD WASTE, DUE TO ITS LOW TOXICITY. THE HYPOTHESIS THAT AN OPRIA FOR PCDDS WILL ALLOW DIRECT ANALYSIS OF THESE POLLUTANTS IN ORGANIC SOLVENT SAMPLE ETRACTS WILL BE TESTED. IF SUCCESSFUL, THIS TECHNOLOGY WILL SIGNIFICANTLY REDUCE THE COST AND TIME REQUIRED TO SCREEN WATER, SOIL, AND OTHER ENVIRONMENTAL SAMPLES POTENTIALLY CONTAMINATED WITH PCDDS.

Principal Investigator:

Gary S Bignami
8084865333

Business Contact:

Small Business Information at Submission:

Hawaii Biotechnology Group Inc
99-193 Aiea Heights Drive Aiea, HI 96701

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No