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TAXOL PURIFICATION BY MONOCLONAL ANTIBODY IMMUNOADSORBENTS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19077
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
19077
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HAWAII BIOTECH, INC.
99-193 AIEA HEIGHTS DRIVE, SUITE 200 AIEA, HI -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1993
Title: TAXOL PURIFICATION BY MONOCLONAL ANTIBODY IMMUNOADSORBENTS
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

THIS PHASE I PROJECT IS DESIGNED TO TEST THE FEASIBILITY OF PURIFYING TAXOL BY IMMUNOAFFINITY CHROMATOGRAPHY. THE USE OF THIS TECHNIQUE FOR PURIFICATION OF A LOW MOLECULAR WEIGHT COMPOUND WITH LOW AQUEOUS SOLUBILITY HAS NOT BEEN REPORTED TO DATE. TAXOL-SPECIFIC MONOCLONAL ANTIBODIES WILL BE SELECTED FOR FURTHER STUDY ON THE BASIS OF THEIR AFFINITY FOR TAXOL AND THEIR ABILITY: (I) TO BIND TAXOL IN AQUEOUS BUFFER CONTAINING 10-20% ORGANIC SOLVENT (II) TO RELEASE TAXOL IN AQUEOUS BUFFER CONTAINING HIGHER CONCENTRATIONS OF THE SAME ORGANIC SOLVENT SELECTED TAXOL-SPECIFIC MONOCLONAL ANTIBODIES (MABS) WILL BE IMMOBILIZED ON SUITABLE SOLID PHASE MATRICES. INCREASING CONCENTRATIONS OF ORGANIC SOLVENT IN AQUEOUS BUFFER, AND HIGH AND LOW PH WILL BE EVALUATED FOR THEIR ABILITY TO DISRUPT BINDING OF TAXOL BY ANTIBODY WITHOUT DENATURING THE MAB. THE GOAL OF PHASE I IS THE DEVELOPMENT OF A BENCH SCALE PROCESS CAPABLE OF SEPARATING TAXOL AND CEPHALOMANNINE.

Principal Investigator:

Paul G Grothaus
8084865333

Business Contact:

Small Business Information at Submission:

Hawaii Biotechnology Group Inc
99-193 Aiea Heights Drive Aiea, HI 96701

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No