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CLONING AND STUDIES OF NEUROSPORA ALKALINE PROTEASE

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
21632
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
21632
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HAWAII BIOTECH, INC.
99-193 AIEA HEIGHTS DRIVE, SUITE 200 AIEA, HI -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1993
Title: CLONING AND STUDIES OF NEUROSPORA ALKALINE PROTEASE
Agency: NSF
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE DEVELOPMENT OF MICROBIAL SYSTEMS FOR EXPRESSION OF HETEROLOGOUS PROTEINS HAS ENABLED THE ISOLATION OF GREATER AMOUNTS OF SOURCE PROTEINS, OFTEN IN GREATER PURITY THAN IS POSIBLE FROM THE ORIGINAL SOURCE. PROTEINS SO EXPRESSED HAVE APPLICATIONS RANGING FROM INDUSTRIAL USE, THROUGH PROTEIN BIOCHEMISTRY RESEARCH, TO HUMAN THERAPEUTICS. RESEARCHERS AT HAWAII BIOTECHNOLOGY GROUP HAVE DEMONSTRATED HETEROLOGOUS PROTEIN EXPRESSION IN NEUROSPORA CRASSA USING TWO PROMOTERS-THE GLUCOSE REPRESSIBLE PROMOTER OF GRG-L AND THE CONSTITUTIVE PROMOTER OF THE BETA-TUBULIN GENE. NEUROSPORA TRANSFORMANTS EXPRESSING A BOVINE PREPROCHYMOSIN CDNA ACCUMULATE SOLUBLE, ENZYMATICALLY ACTIVE MATURE CHYMOSIN IN THE CULTURE MEDIUM. ONE IMPORTANT ELEMENT OF HETEROLOGOUS PROTEIN EXPRESSION SYSTEMS IS THE APPROPRIATE HOST STRAIN, FOR WHICH PROTEASE-DEFICIENT MUTANTS ARE OFTEN SUPERIOR. NEUROSPORA SECRETES AN ALKALINE PROTEASE IN THE PRESENCE OF PROTEIN IN THE CULTURE MEDIUM WHEN ALSO STARVED FOR CARBON, NITROGEN, OR SULFUR. WHEN INDUCED, THE ALKALINE PROTEASE COMPRISES 40 PERCENT OF SECRETED PROTEINS. TO DEVELOP BOTH AN IMPROVED HOST AND ADDITIONAL EXPRESSION VECTORS FOR EXPRESSION OF HETEROLOGOUS PROTEINS, RESEARCHERS ARE CLONING CDNA AND THE GENOMIC LOCUS ENCODING THE N. CRASSA SECRETED ALKALINE PROTEASE. THESE CLONES WILL BE USED TO CREATE ALKALINE PROTEASE-DEFICIENT DISRUPTION MUTATIONS AND TO ADD THE PROTEASE PROMOTER AND SECRETION SIGNAL PEPTIDE TO THE EXPRESSION VECTOR REPERTOIRE.

Principal Investigator:

John Marshall Ivy
8084865333

Business Contact:

Small Business Information at Submission:

Hawaii Biotechnology Group Inc
99-193 Aiea Heights Dr Aiea, HI 96701

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No