Expression of Single-chain FV in Neurospora Crassa

Award Information

Agency: Department of Health and Human Services	Branch: N/A	Award ID: 21929	Program Year/Program: 1993 / SBIR
Agency Tracking Number: 21929	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: HAWAII BIOTECH, INC.
99-193 AIEA HEIGHTS DRIVE, SUITE 200 AIEA, HI 96701-3900
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Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No

Phase 1: Fiscal Year: 1993

Title: Expression of Single-chain FV in Neurospora Crassa

Agency: HHS

Contract: N/A

Award Amount: $50,000.00

Abstract:
The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. Secretion is a key element in their attraction; secretion provides the opportunity for complex proteins to fold and be processed properly and secretion will facilitate purification. Proper folding and processing, simplified purification, and inexpensive culturing translate into less expensive production. We have demonstrated secretion and processing of an enzymatically active mammalian protein (preprochymosin) from Neurospora crassa, and we will test the feasibility of expressing a single chain antibody fragment in Neurospora. The use of Neurospora to express single chain antibodies is novel.

Principal Investigator:
Eileen T. Nakano
8084865333

Business Contact:

Small Business Information at Submission:

Hawaii Biotechnology Group,
99-193 Aiea Heights Drive Aiea, HI 96701

EIN/Tax ID:

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No