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Anti-Flavivirus Immunotherapeutics

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
54180
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
1R43AI047610-01A1
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HAWAII BIOTECH, INC.
99-193 AIEA HEIGHTS DRIVE, SUITE 200 AIEA, HI -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: Anti-Flavivirus Immunotherapeutics
Agency: HHS
Contract: N/A
Award Amount: $172,415.00
 

Abstract:

DESCRIPTION (provided by the applicant): There are 100 million cases of dengue infection each year with 250,000 cases of the life-threatening Dengue Hemorrhagic Fever/Dengue Shock Syndrome (DHS/DSS). Treatment options are limited to symptoms management. Passive immunoprophylaxis and immunotherapy are standard treatments for a number of viral diseases. However, the undefined nature of hyperimmune serum is unsuitable for dengue virus disease treatment. To provide a defined reagent treatment for severe disease, we propose to examine the efficacy of using cobra venom factor linked antibodies to the viral structural envelope (E) and the viral first non-structural (NS1) proteins as immunotherapeutic agents. In Phase I a combinatorial human Fab library will be constructed, and Fab fragments which recognize either E or NS1 proteins will be identified. These fragments will be evaluated for cross-reactivity, non-overlapping epitope recognition and affinity. All Fab fragments will be conjugated to cobra venom factor and evaluated for complement mediated cytolysis and anti-E conjugates for reduction in virus infectivity. To further evaluate the immunoprophylaxis potential of the conjugates, in vivo mouse challenge studies will also be performed. In Phase II, F(ab)2-humanized CVF conjugates will be expressed in mammalian cells and their efficacy evaluated in mice and non-human primates. PROPOSED COMMERCIAL APPLICATION: The dengue virus complex specific anti-E and NS1 Fab fragments identified in Phase I research have the potential to relieve severe dengue disease symptoms. More than 250,000 annual cases of severe dengue disease are reported each year.

Principal Investigator:

Eileen T. Nakano

Business Contact:


8084865333
STAFF@HIBIOTECH.COM
Small Business Information at Submission:

HAWAII BIOTECHNOLOGY GROUP, INC.
99-193 AIEA HEIGHTS DR, STE 236 AIEA, HI 96701

EIN/Tax ID: 990214983
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No