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OPTIMIZING MULTIPARAMETER FLOW CYTOMETRY OF TUMOR CELLS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7139
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7139
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
HOWARD M. SHAPIRO, MD, P.C.
283 HIGHLAND AVE. WEST NEWTON, MA 02465 2513
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: OPTIMIZING MULTIPARAMETER FLOW CYTOMETRY OF TUMOR CELLS
Agency: HHS
Contract: N/A
Award Amount: $44,000.00
 

Abstract:

FLOW CYTOMETRY OF SUCH PARAMETERS AS DNA, RNA, SURFACE AND/OR INTERNAL ANTIGEN, AND TOTAL PROTEIN CONTENT OF TUMOR CELLS OR NUCLEI IS WIDELY USED IN CANCER RESEARCH AND IS FINDING INCREASING CLINICAL APPLICATION. USEFUL INFORMATIONABOUT THE BIOLOGY OF NORMAL AND ABNORMAL CELLULAR DEVELOPMENT, THE PERTURBATION OF CELL FUNCTION BY THERAPEUTIC AGENTS, AND THE BIOLOGIC BEHAVIOR OF TUMORS IN PATIENTS, OBTAINED FROM ONE-AND TWO-PARAMETER FLOW CYTOMETRY, HAS LED INVESTIGATORS TO ATTEMPT MORE SOPHISTICATED STUDIES IN WHICH DNA, RNA, ONE OR MORE CELLULAR ANTIGENS, AND LIGHT SCATTERING OR EXTINCTION ARE MEASURED SIMULTANEOUSLY. THE MULTIPLE-BEAM APPARATUS AND MANY OF THE STAINING PROCEDURES USABLE FOR SUCH ANALYSES HAVE BEEN DEVELOPED; HOWEVER, THE TECHNIQUES HAVE REMAINED INACCESSIBLE TO MANY INVESTIGATORS BECAUSE OF THE DIFFICULTY AND COST OF IMPLEMENTING THEM ON CONVENTIONAL COMMERCIAL FLOW CYTOMETERS. THE PRESENT STUDY WILL EXAMINE THE PERFORMANCE OF TWO NEWER FLOW CYTOMETERS USING LOW-POWER LASER LIGHT SOURCES IN MULTIPARAMETER ANALYSIS OF DNA, RNA, AND IMMUNOFLUORESCENCE AND/OR PROTEIN CONTENT IN CELLS DERIVED FROM HUMAN TUMORS. THESE INSTRUMENTS, POTENTIALLY MUCH MORE AFFORDABLE AND EASIER TO USE THAN EXISTING SYSTEMS, COULD MAKE IT POSSIBLE FOR MANY MORE LABORATORIES TO PURSUE CLINICAL AND EXPERIMENTAL APPLICATIONS OF MULTIPARAMETER FLOW CYTOMETRY.

Principal Investigator:

Howard m shapiro md
6179656044

Business Contact:

Small Business Information at Submission:

Howard M Shapiro Md Pc
283 Highland Avenue West Newton, MA 02165

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No