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OPTIMIZATION AND EVALUATION OF AN ACROSOME ASSAY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11193
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11193
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Humagen Inc
1500 Avon St Ext Charlottesville, VA 22901
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: OPTIMIZATION AND EVALUATION OF AN ACROSOME ASSAY
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE ABILITY TO MEASURE AN ACROSOME REACTION IN HUMAN SPERM MAY LEAD TO NEW DIAGNOSES AND THERAPY FOR MALE INFERTILITY. A NUMBER OF TECHNIQUES HAVE BEEN DESCRIBED, BUT NONE ARE ROUTINELY USED IN A CLINICAL SEMEN ANALYSIS. THE OBJECTIVE OF THIS RESEARCH IS TO OPTIMIZE THE CONDITIONSFOR AN INDIRECT IMMUNOFLUORESCENCE ASSAY USING EXISTING MONOCLONAL ANTIBODIES, T6 AND MHS-10, TO QUANTITATE ACROSOME-REACTED SPERM. ASSAYS WILL BE DEVELOPED FOR DETERMINING THE ACROSOME STATUS OF A FRESH EJACULATE AS WELLAS FOR MEASURING THE ACROSOME-INDUCIBLE POPULATION IN A SEMEN SPECIMEN. THE ASSAY WILL BE OPTIMIZED FOR MINIMAL DAY-TO-DAY AND DONOR-TO-DONOR VARIABILITY. THE INDIRECT IMMUNOFLUORESCENCE ASSAY WILL ALSO BE EVALUATED USING AN ACROSOME INDUCTION TECHNIQUE THAT HAS BEEN EXTENSIVELY CORRELATED WITH IN VITRO FERTILIZATION AND PREGNANCY RATES. IF AN ACROSOME-REACTED POPULATION CAN BE CONSISTENTLY DETECTED, THE ASSAY WILL BE MOVED INTO CLINICAL TRIALS AT BAYLOR COLLEGE OF MEDICINE TO EVALUATE SPERM FROM INFERTILE PATIENTS.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Humagen Inc
1500 Avon St Ext Charlottesville, VA 22901

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No