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Phase plate holder for transmission electron microscopy

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
93800
Program Year/Program:
2009 / SBIR
Agency Tracking Number:
GM085841
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Hummingbird Scientific, Llc
2610 Willamette Drive NE Suite A Lacey, WA 98516-1329
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2009
Title: Phase plate holder for transmission electron microscopy
Agency: HHS
Contract: 1R43GM085841-01A1
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): The objective of our application is to develop hardware and procedures that will allow researchers to practically utilize phase plates in the back focal plane of the objective lens in a Transmission Electron Microscope. Use of precisely positioned phase plates can increase resolution in electron tomography, and also increase throughput when using the single-particle approach. Our specific aim is to provide a phase plate system that will include a modified objective apert ure holder that incorporates (1) a piezo positioning system, (2) a heater to avoid contamination, and (3) an electrical feed-through for use with electrostatic phase plates. Simply exchanging the original objective aperture holder with the Hummingbird phas e plate holder will allow nearly any cryo-EM to be equipped with a phase plate. The cost of this holder will be substantially lower than any other technology that can yield a similar improvement in imaging of frozen-hydrated specimens. In close collaborati on with the Resource for the Visualization of Biological Complexity (RVBC) at the Wadsworth Center's NIH Biotechnological Resource, we will develop a new phase plate holder, demonstrate its use, and publish the results. It is our intent to develop and comm ercialize a cost effective system that will enable these techniques to become widely available to the biology research community. This proposal will develop tools that allow biologists to get a clearer picture of the structure and function of the cell. Thi s knowledge is fundamental to innumerable areas of medical research and practice.

Principal Investigator:

Norman J. Salmon
3602522737
NORMAN@HUMMINGBIRDSCIENTIFIC.COM

Business Contact:

Norman Salmon
norman@hummingbirdscientific.com
Small Business Information at Submission:

HUMMINGBIRD SCIENTIFIC, LLC
HUMMINGBIRD PRECISION MACHINE CO 8300 28TH COURT NE STE 200 LACEY, WA 98516

EIN/Tax ID: 120197174
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No