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Supplement for System for generation of frozen-hydrated samples using the…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
93814
Program Year/Program:
2009 / SBIR
Agency Tracking Number:
GM086964
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Hummingbird Precision Machine Co
2610 Willamette Drive NE Suite A Lacey, WA 98516-1329
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2009
Title: Supplement for System for generation of frozen-hydrated samples using the Focused
Agency: HHS
Contract: 1R43GM086964-01A1
Award Amount: $350,000.00
 

Abstract:

DESCRIPTION (provided by applicant): Transmission electron microscope (TEM) tomography provides three-dimensional images at the nanometer scale. It is a mainstream technique in biological electron microscopy, and is rapidly finding applications in the semi conductor and nanotechnology industries. In biology, scientists are interested in producing faithful tomograms of frozen-hydrated samples, since specimens prepared in this way are considered to represent the native in vivo structure. In the case of typical mammalian cells and tissue, such specimens are usually high- pressure frozen, and then prepared for electron tomography using cryo-ultramicrotomy. However the difficulties of cryo-ultramicrotomy, and the artifacts that are unavoidably created by mechanica l sectioning, have discouraged most laboratories from attempting study of frozen-hydrated cells and tissue by electron tomography. Preliminary work by our Wadsworth collaborators has shown that use of a cryo-FIB system avoids the artifacts and difficulties of cryo-ultramicrotomy, and offers additional advantages in preparing frozen-hydrated specimens. PUBLIC HEALTH RELEVANCE: This proposal will develop tools that allow biologists to get a clearer picture of the structure and function of the cell. This knowl edge is fundamental to innumerable areas of medical research and practice.

Principal Investigator:

Norman J. Salmon
3602522737
NORMAN@HUMMINGBIRDSCIENTIFIC.COM

Business Contact:

Norman J. Salmon
norman@hummingbirdscientific.com
Small Business Information at Submission:

HUMMINGBIRD SCIENTIFIC, LLC
HUMMINGBIRD PRECISION MACHINE CO 8300 28TH COURT NE STE 200 LACEY, WA 98516

EIN/Tax ID: 120197174
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No