Not Available Tera Biotechnology Corporation proposes to develop an automated system to screen phage antibody libraries by our parallel selection process. The system we propose could be used to routinely generate antibodies of any specificity without the need for human intervention. For Phase I we… More

Development of single use, freeze-dried PCR reagents for use with the Idaho Technology Ruggedized Advanced Pathogen Identification Device (RAPIDT system) or other commercially available real-time PCR devices is proposed. These reagents will have extendedstability at ambient temperature thus… More

DESCRIPTION (applicant's abstract): Our long-term goal is to advance techniques and instrumentation for rapid, automated analysis of nucleic acids during amplification. Our approach is to add fluorescent probes before amplification and analyze the results in real-time,… More

DESCRIPTION (provided by applicant): Real-time PCR has greatly improved the ease, accuracy and precision of quantitative PCR by allowing reaction kinetics to be observed, measured and recorded as PCR is occurring. We have successfully commercialized the LightCycler, a real-time… More

DESCRIPTION (provided by applicant): Real-time PCR has greatly improved the ease, accuracy and precision of quantitative PCR by allowing reaction kinetics to be observed, measured and recorded as PCR is occurring. We have successfully commercialized the LightCycler, a real-time… More

"The Idaho Technology RAPID is currently the only fieldable DNA-based system available for monitoring threat agents such as Bacillus anthracis and Yersinia pestis. It has been deployed throughout the Air Force and other branches of the military with a highlevel of operational success. Typical PCR… More

"The Idaho Technology RAPID is currently the only fieldable DNA-based system available for monitoring threat agents such as Bacillus anthracis and Yersinia pestis. It has been deployed throughout the Air Force and other branches of the military with a highlevel of operational success. Typical PCR… More

DESCRIPTION (provided by applicant): We will develop software for automated pathogen detection and quantification using data from PCR experiments. Automated pathogen detection using data from a PCR experiment requires software to determine whether DNA from the pathogen is present or absent in a… More

DESCRIPTION (provided by applicant): Wilson Disease (WD) is an autosomal recessive disorder of copper metabolism. Prospective screening for WD has been proposed however a sensitive and specific biochemical genetic assay was not available and primary molecular analysis was not feasible. WD may be the… More

DESCRIPTION (provided by applicant): Our objective is to provide a homogeneous, closed-tube system for DNA mutation scanning on a high-throughput platform. For many complex genetic diseases, it is very difficult and expensive to screen for all possible mutations that may cause the disease. We… More

DESCRIPTION (provided by applicant): Polymerase chain reaction (PCR) has become the method of choice for rapid diagnosis of infectious agents. To identify an infectious agent by molecular means requires a panel of PCR assays. So called "multiplex PCR" concurrently assays for multiple targets in a… More

DESCRIPTION (provided by applicant): Our objective is to provide a closed-tube system for rapid PCR, mutation scanning and genotyping that does not require fluorescently-labeled probes. For many genetic diseases, it is difficult and expensive to screen for all possible mutations that may cause the… More

DESCRIPTION (provided by applicant): Our objective is to provide a closed-tube system for rapid PCR, mutation scanning and genotyping that does not require fluorescently-labeled probes. For many genetic diseases, it is difficult and expensive to screen for all possible mutations that may cause the… More

DESCRIPTION (provided by applicant): Urea Cycle Disorders (UCDs) are metabolic diseases disrupting ureagenesis. Unequivocal and rapid diagnosis of UCDs is difficult because hyperammonemia, the primary biochemical phenotype, is not specific to UCDs being observed in broad categories of metabolic… More

DESCRIPTION (provided by applicant): Completion of the human genome and the advent of expression profiling have permitted comprehensive characterization of the transcriptional signatures of different disease states. Quantitative fluorescent real-time polymerase chain reaction-based methods have… More

DESCRIPTION (provided by applicant): Diseases involving the urea cycle are clinically manifest by symptoms diverse as neonatal hyperammonemic coma to postpartum psychosis. Hyperammonemia is the primary phenotype of urea cycle defects but differentiating individual gene deficiencies within the urea… More

DESCRIPTION (provided by applicant): In 1958, Dr Robert Guthrie was approached by Dr Robert Warner seeking aid to develop a better means to measure phenylalanine in the blood of newborns. Of this interaction was born the bacterial inhibition assay and the beginning of population-based newborn… More

Not avaiable.

DESCRIPTION (provided by applicant): The objective of this Fast-Track STTR is to develop a simple, inexpensive, closed-tube PCR method for genotyping and sequence variant scanning. Instead of covalently-labeled probes, one of two primers includes a tail w ith a probe element. After asymmetric PCR,… More

DESCRIPTION (provided by applicant): The objective of this Fast-Track STTR is to develop a simple, inexpensive, closed-tube PCR method for genotyping and sequence variant scanning. Instead of covalently-labeled probes, one of two primers includes a tail w ith a probe element. After asymmetric PCR,… More

DESCRIPTION (provided by applicant): Cystic fibrosis (CF) is an autosomal recessive, life-shortening disease that affects ~30,000 patients in the U.S., while there are estimated 10 million symptomless carriers in the U.S. (www.cff.org). Approximately one in 2,500-3,300 newborns in the U.S. have… More

DESCRIPTION (provided by applicant): Immunocompromised patients including those who have undergone hematopoietic stem cell (HSCT) and solid organ (SOT) transplants are at markedly increased risk for severe disease caused by respiratory pathogens. These pat ients have greater susceptibility to a… More