Development of the nanoDETECT Integrated Pathogen Detection System
Sample preparation is extremely important to food borne pathogen detection because food matrices can severely inhibit nucleic acid amplification based detection methods. Samples must typically be pre-enriched to provide adequate numbers of target cells for detection. In addition, current zero-tolerance levels necessitate a very rigorous detection level. IMS is an attractive alternative to preenrichment since it can both remove target cells from complex matrices (such as food samples) and also concentrate target cells for rapid detection. The sample preparation cartridge will complement the existing breadboard instrumentation of Illuminarias nanoDETECT platform for the detection of the target pathogens from contaminated food samples.
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