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STANDARDIZATION OF ASSAY FOR ANTIBODIES TO ISLET CELLS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4882
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
4882
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
IMMCO DIAGNOSTICS
IMMCO DIAGNOSTICS 60 PINEVIEW DRIVE BUFFALO, NY 14228
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1988
Title: STANDARDIZATION OF ASSAY FOR ANTIBODIES TO ISLET CELLS
Agency: HHS
Contract: N/A
Award Amount: $350,000.00
 

Abstract:

THE TEST FOR ISLET CELL CYTOPLASMIC ANTIBODIES (ICA) IS A GOOD PREDICTOR OF INDIVIDUALS AT RISK TO DEVELOP TYPE I DIABETES AND THUS HAS POTENTIAL VALUE IN IDENTIFYING CANDI- DATES FOR FUTURE MEASURES AIMED AT PREVENTING THE DISEASE. HOWEVER, PROBLEMS WITH STANDARDIZATION LIMIT THE RELIABILITYOF THE ICA ASSAY. THE GOAL OF THIS PROJECT IS TO MAKE AVAILABLE A STANDARDIZED, AND HENCE MORE RELIABLE, SYSTEM FOR DETECTING ICA. SPECIFIC OBJECTIVES ARE TO: (1) ESTABLISH A PROTOCOL FOR PROCURING DONOR PANCREAS THAT RESULTS IN OPTIMAL REACTIVITY AND RETENTION OF TISSUE MORPHOLOGY; (2) ESTABLISH THE MOST SENSITIVE AND REPRODUC- IBLE FLUORESCENT INDICATOR SYSTEM FOR VISUALIZING THE ICA REACTION; (3) MAXIMIZE THE STABILITY OF ISLET CELL ANTIGENS IN TISSUE SECTIONS; AND (4) DEVELOP A SEPARATE PARALLEL SYSTEM THAT ALLOWS THE USER TO ASSESS HIS SKILLS IN PERFORMING, READING, AND INTERPRETING THE ICA TEST. THESE AIMS WILL BE ADDRESSED BY: (1) DOCUMENTING TIME LIMITS FOR TISSUE PROCESSING BEYOND WHICH REACTIVITY AND HISTOLOGY DETERIORATE AND EXAMINING PANCREAS PERFUSION AS A MEANS OF EXTENDING THESE TIME LIMITS; (2) COMPARING FIVE FLOURESCENT INDICATORS FOR SPECIFICITY, SENSITIVITY, AND REPRODUCIBILITYIN THE ICA ASSAY; (3) COMPARING SHELF-LIVES OF PANCREAS SECTIONS AFTER VARIOUS MODES OF FIXATION; AND (4) DEVELOPINGTHE COMPONENTS OF A USER STANDARDIZATION KIT. THIS RESEARCHWILL LAY THE GROUNDWORK FOR PHASE II DEVELOPMENT OF A COMMERCIALLY MARKETABLE ICA ASSAY PACKAGE.

Principal Investigator:

Jay G Huang
Principal Investigator
7168765672

Business Contact:

Small Business Information at Submission:

Immco Diagnostics
963 Kenmore Avenue Buffalo, NY 14223

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No