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THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4693
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4693
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Immunex Corp
51 University Street Seattle, WA 98101
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA CLONING, EXPRESSION) OF MURINE AND HUMAN GENES CODING FOR A GROWTH FACTOR FOR PREMATURE B- LYMPHOCYTES (PRE-BC-GF).
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE LONG-TERM OBJECTIVE OF THIS PROJECT IS THE MOLECULAR CHARACTERIZATION (CDNA CLONING, EXPRESSION) OF MURINE AND HUMAN GENES CODING FOR A GROWTH FACTOR FOR PREMATURE B- LYMPHOCYTES (PRE-BC-GF). THE APPROACH WILL BE TO CONSTRUCT A CDNA LIBRARY FROM SIZED, ACTIVE, PRE-BC-GF MRNA AND TO ISOLATE A FULL-LENGTH PRE-BC-GF CDNA BY HYBRIDIZATION WITH AN OLIGONUCLEOTIDE PROBE DEDUCED FROM PROTEIN SEQUENCE INFORMATION DERIVED FROM PURIFIED PRE-BC-GF PROTEIN. DURING PHASE I, PRE-BC-GF ACTIVITY WILL BE PURIFIED FROM SUPERNATES PRODUCED BY A TRANSFECTED/TRANSFORMED MURINE STROMAL BONE MARROW CELL LINE (SV CELL LINE) RECENTLY DEVELOPED IN OUR LABORATORY. THE MRNA CODING FOR PRE-BC-GF ACTIVITY IN VITRO WILL ALSO BE PREPARED FROM THE SV CELL LINE AND SIZE FRACTIONATED IN PREPARATION FOR CDNA CLONING. ONCE MURING PRE-BC-GF CDNAS ARE ISOLATED, THEY WILL BE EXPRESSED IN APPROPRIATE YEAST AND E. COLI VECTORS. RECOMBINANT PRE-BC-GF WILL THEN BE TESTED FOR BIOLOGICAL ACTIVITY IN MURINE MODEL SYSTEMS OF B-CELL DEFICIENCY. FINALLY, MURINE CDNAS FOR PRE-BC-GF WILL BE USED AS PROBES FOR IDENTIFICATION BY SOUTHERN BLOT HYBRIDIZATION OF ANALOGOUS HUMAN CDNAS. PRE-BC-GF COULD BE OF VALUE IN CLINICAL RESTORATION OF ANTIBODY RESPONSIVENESS IN CASES OF VIRAL, CHEMOTHERAPEUTIC, OR RADIATION INDUCED IMMUNE SUPPRESSION.

Principal Investigator:

Steven gillis
PRINCIPAL INVESTIGATOR
2065870430

Business Contact:

Small Business Information at Submission:

Immunex Corp
51 University Street Seattle, WA 98101

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No