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DNA PROBES FOR DIAGNOSIS OF GONOCOCCAL DISEASE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7877
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
7877
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Inland Laboratorytories
2600 Stemmons Freeway Dallas, TX 75207
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: DNA PROBES FOR DIAGNOSIS OF GONOCOCCAL DISEASE
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

DEVELOPMENT OF AN EFFICACIOUS AND COST-EFFECTIVE DIAGNOSTIC TEST FOR NEISSERIA GONORRHOEAE INFECTION IS THE GOAL OF THIS PROJECT. ALTHOUGH CURRENT DIAGNOSTIC METHODS ARE SENSITIVE AND SPECIFIC, THEY ARE TIME CONSUMING AND COSTLY. BECAUSE OF THE ENORMOUS MORBIDITY OF GONOCOCCAL INFECTION IN THE U.S., THE POTENTIAL FOR COMMERCIAL APPLICATION OF SUCH A TEST IS EXCELLENT. THIS PHASE I PROPOSAL EXAMINES THE FEASIBILITY OF TWO DNA "PROBES" AS DIAGNOSTIC REAGENTS. ONE WILL BE BASED ON THE DNA SEQUENCE OF A 26 BASEPAIR REPETITIVE ELEMENT FOUND IN THE GENOMES OF NEISSERIA SPP. A 21 BASE OLIGONUCLEOTIDE WILL BE COMMERCIALLY SYNTHESIZED AND ENZYMATICALLY BIOTINYLATED. THE SECOND PROBE WILL BE A SINGLE STRANDED DNA FRAGMENT OF THE GONOCOCCAL OUTER MEMBRANE PROTEIN III GENE. THIS PROBE WILL BE BIOTINYLATED CHEMICALLY. A RESTRICTION FRAGMENT OF THIS GENE DEMONSTRATING THE BEST SPECIES SPECIFICITY WILL BEDETERMINED BY HYBRIDIZATION ANALYSIS WITH GENOMIC DNA FROM SEVERAL NEISSERIA SPP. BOTH PROBES WILL BE EXAMINED FOR SENSITIVITY USING PURIFIED GONOCOCCAL DNA AND HYBRIDIZATION ANALYSIS ON NITROCELLULOSE. BIOTINYLATED DNA PROBES WILL BE DETECTED WITH COMPLEXES OF STREPTAVIDIN AND BIOTINYLATED ALKALINE PHOSPHATASE OR UREASE. PROBES WILL BE CONSIDERED FOR FURTHER DEVELOPMENT (PHASE II), IF THEIR LEVEL OF SENSITIVITY ALLOWS DETECTION OF LESS THAN TEN THOUSAND GONOCOCCI WITH INSIGNIFICANT BACKGROUND "NOISE".

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Inland Laboratories
10125 Metropolitan Austin, TX 78758

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No