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Enhancing site-specific chromosomal integration in clostridia

Award Information

Agency:
Department of Energy
Branch:
N/A
Award ID:
94448
Program Year/Program:
2009 / SBIR
Agency Tracking Number:
91201
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Elcriton
15 Reads Way Suite 106 New Castle, DE 19720-0000
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: Yes
 
Phase 1
Fiscal Year: 2009
Title: Enhancing site-specific chromosomal integration in clostridia
Agency: DOE
Contract: DE-FG02-09ER85341
Award Amount: $99,682.00
 

Abstract:

The bacteria genus, Clostridium, includes many species that are naturally capable of degrading cellulose and producing valuable chemicals and fuel alcohols. Unfortunately the genetic engineering tools for developing improved strains are limited for all clostridia. This project will develop novel approaches for enhancing chromosomal integration in all clostridia species. With an enhanced recombination system for clostridia, it would become possible to quickly knock out and knock in genes, allowing new strains with superior production capabilities to be quickly engineered. Phase I will demonstrate the feasibility of improving site-specific chromosomal integration via recombinant over-expression of native and heterologous proteins involved in homologous recombination. Phase I will focus on the over-expression of individual proteins, and Phase II will build upon those results by studying the over-expression of combinations of proteins. Commercial Applications and other Benefits as described by the awardee The technology should significantly accelerate strain development in solventogenic and other clostridia species, leading to the microbial conversion of renewable carbohydrates to chemicals and fuels. Such a development would aid our nation┬┐s goals of developing sustainable and greener technologies for the production of chemicals and transportation fuels

Principal Investigator:

Bryan Tracy
Mr.
8649215146
bryantracy2009@u.northwestern.edu

Business Contact:

Danielle Nolen
Ms.
3025283205
dnolen68@gmail.com
Small Business Information at Submission:

Elcriton
15 Innovation Way #288 Newark, DE 19711

EIN/Tax ID: 314749182
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No