An Official Website of the United States Government
RAPID, SINGLE MOLECULAR DNA SEQUENCING NANOPORE DEVICE
- Small Business Information
-
Woman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
- Phase 1
-
Fiscal Year: 2002Title: RAPID, SINGLE MOLECULAR DNA SEQUENCING NANOPORE DEVICEAgency: HHSContract: 2R44HG002327-02Award Amount: $0.00
Abstract:
DESCRIPTION (provided by applicant): The objective of this Phase II program is to complete the development of a commercializable solid state nanopore device for the accurate high speed sequencing of DNA. To achieve this goal a manufacturable device must be designed and fabrication processes established that result in a unit with extremely small, well controlled diameter orifices of approximately 2 nm through a multi-layered thin film sandwich of insulators and conductors. The Phase I portion of this program established the methodology of: creating a thin (30 nm) SiN film over a 20 um square window in a 1 cm square silicon frame; Focused Ion Beam (FIB) milling a nanopore; subsequent deposition of a multi-layer thinfilm sandwich of conductor and insulating layers on the surface of SiN film; and building a cartridge to hold and seal the nanopore chip between two liquid cells, and provide electrical terminations for measuring the changes in current, potential, or capacitance resulting in the passage of a DNA molecule chain through the nanopore. It was found that FIB milling produced a nanopore that was too wide and lacked the required sensitivity to map DNA molecules passing through the nanopore.. A new approach, TEM drilling will be used to achieve nanopores of 2 nm or smaller. Additional work will be invested in optimizing the leads and instrumentation, i.e., patch clamp amplifiers to achieve improved signal-to-noise ratios and increased detection sensitivity.Small Business Information at Submission:ADVANCED RESEARCH CORPORATION
ADVANCED RESEARCH CORPORATION 815 14TH AVE SE MINNEAPOLIS, MN 55414EIN/Tax ID: 411563821DUNS: N/ANumber of Employees: N/AWoman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
Award Information
|
Agency: Department of Health and Human Services |
Branch: N/A |
Award ID: 60223 |
Program Year/Program: 2002 / SBIR |
|
Agency Tracking Number: HG002327 |
Solicitation Year: N/A |
Solicitation Topic Code: N/A |
Solicitation Number: N/A |