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RAPID, SINGLE MOLECULAR DNA SEQUENCING NANOPORE DEVICE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60223
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
HG002327
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Advanced Research Corporation
4459 White Bear Parkway White Bear Lake, MN -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2002
Title: RAPID, SINGLE MOLECULAR DNA SEQUENCING NANOPORE DEVICE
Agency: HHS
Contract: 2R44HG002327-02
Award Amount: $1,207,904.00
 

Abstract:

DESCRIPTION (provided by applicant): The objective of this Phase II program is to complete the development of a commercializable solid state nanopore device for the accurate high speed sequencing of DNA. To achieve this goal a manufacturable device must be designed and fabrication processes established that result in a unit with extremely small, well controlled diameter orifices of approximately 2 nm through a multi-layered thin film sandwich of insulators and conductors. The Phase I portion of this program established the methodology of: creating a thin (30 nm) SiN film over a 20 um square window in a 1 cm square silicon frame; Focused Ion Beam (FIB) milling a nanopore; subsequent deposition of a multi-layer thinfilm sandwich of conductor and insulating layers on the surface of SiN film; and building a cartridge to hold and seal the nanopore chip between two liquid cells, and provide electrical terminations for measuring the changes in current, potential, or capacitance resulting in the passage of a DNA molecule chain through the nanopore. It was found that FIB milling produced a nanopore that was too wide and lacked the required sensitivity to map DNA molecules passing through the nanopore.. A new approach, TEM drilling will be used to achieve nanopores of 2 nm or smaller. Additional work will be invested in optimizing the leads and instrumentation, i.e., patch clamp amplifiers to achieve improved signal-to-noise ratios and increased detection sensitivity.

Principal Investigator:

Matthew Dugas
6123312211
mdugas@gotoarc.com

Business Contact:

Judy Dugas
6123312211
JDUGAS@GOTOARC.COM
Small Business Information at Submission:

ADVANCED RESEARCH CORPORATION
ADVANCED RESEARCH CORPORATION 815 14TH AVE SE MINNEAPOLIS, MN 55414

EIN/Tax ID: 411563821
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No