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PRODUCTION OF A HORMONE RECEPTOR COMPETITIVE ANTAGONIST

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7939
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
7939
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Integrated Genetics Inc
1 Mountain Rd Framingham, MA 01701
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: PRODUCTION OF A HORMONE RECEPTOR COMPETITIVE ANTAGONIST
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

RECENT RESEARCH IN REPRODUCTIVE ENDOCRINOLOGY HAS DEMONSTRATED THE IMPORTANCE OF FOLLICLE STIMULATING HORMONE (FSH) IN THE REGULATION OF GAMETOGENESIS AND GONADAL STEROIDPRODUCTION. IT HAS BEEN SHOWN THAT N-LINKED OLIGOSACCHARIDES, ATTACHED TO ASPARAGINE RESIDUES AT SPECIFIC SITES ALONG THE MOLECULE, ARE ESSENTIAL FOR POST-RECEPTOR TARGET CELL ACTIVATION BUT NOT RECEPTOR BINDING. THESE SUGARS ALSO PLAY A ROLE IN DETERMINING THE HALF-LIFE OF THE MOLECULE IN CIRCULATION. WE PLAN TO USE THE TECHNIQUE OF SITE-DIRECTED MUTAGENESIS TO PRODUCE A RECOMBINANT HUMAN FSH MOLECULE THAT WILL HAVE ONE OR MORE OFTHE ASPARAGINE RESIDUES REPLACED WITH A SIMILARLY CHARGED AMINO ACID TO "ERASE" THE SITE OF N-LINKED SUGAR ATTACHMENT.PRELIMINARY STUDIES HAVE SHOWN THAT REMOVAL OF SUGARS FROM THE ALPHA CHAIN SEVERELY DECREASE BIOLOGIC ACTIVITY. REMOVAL OF THESE SUGAR RESIDUES, BY MUTAGENESIS, WILL PRODUCE A LONG ACTING COMPETITIVE ANTAGONIST OF FSH, WHICH, WHEN ADMINISTERED TO HUMANS, WOULD BE EXPECTED TO RESULT IN AN ANOVULATORY STATE OR AZOSPERMIA. A MUTAGENIZED, RECOMBINANT HORMONE MAY BE PRODUCED IN LARGE AMOUNTS, WITH EACH BATCH EXHIBITING UNIFORM BIOCHEMICAL AND BIOLOGICAL PROPERTIES. THEREFORE, THIS PRODUCT WOULD BE AN EXCELLENT CHOICE AS A REVERSIBLE CONTRACEPTIVE IN MEN AND WOMEN.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Integrated Genetics Inc
1 Mountain Rd Framingham, MA 01701

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No