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AUTOMATED LARGE SCALE ANALYSIS OF HUMAN COSMID LIBRARIES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
8028
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
8028
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Integrated Genetics Inc
1 Mountain Rd Framingham, MA 01701
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1988
Title: AUTOMATED LARGE SCALE ANALYSIS OF HUMAN COSMID LIBRARIES
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE OBJECTIVE OF THE PROPOSED RESEARCH PLAN IS TO DEVELOP METHODS TO RAPIDLY SCREEN, ISOLATE, AND CHARACTERIZE COSMID CLONES FROM HUMAN DNA LIBRARIES. IN PHASE I WE WILL ESTABLISH PROCEDURES FOR USING A ROBOTIC WORKSTATION TO AUTOMATE THE SCREENING OF A COSMID LIBRARY PREPARED FROM A SOMATIC CELL HYBRID THAT CONTAINS HUMAN CHROMOSOME 21. WE WILL ALSO OPTIMIZE THE USE OF AN INSTRUMENT THAT CAN DIRECTLY READ AND DIGITIZE BETA EMISSIONS FROM (32)P ISOTOPECONTAINED ON HYBRIDIZED FILTERS TO INCREASE THE EFFICIENCY OF LIBRARY SCREENING. TWO METHODS WILL BE COMPARED THAT RESULT IN RESTRICTION SITE MAP DATA FOR COSMID CLONES ALLOWING OVERLAPS TO BE CONSTRUCTED FROM INDEPENDENTLY ISOLATED COSMIDS. STANDARD PROTOCOLS WILL BE ESTABLISHED FOR GENERATING THIS DATA AND KEY AREAS FOR AUTOMATION OF DATA PROCESSING USING THE DIRECT (32)P DETECTOR WILL BE IDENTIFIED. A LARGE SCALE IMPLEMENTATION OF THESE METHODS DURING PHASE II WILL BEGIN THE CONSTRUCTION OF A COMPLETE PHYSICAL MAP OF CHROMOSOME 21. A MAP OF CHROMOSOME 21 WILL SIGNIFICANTLY AID IN THE UNDERSTANDING OF THE ROLE OF THIS CHROMOSOME AND SPECIFIC GENES IN THE ETIOLOGY AND PATHOGENESIS OF DOWN SYNDROME AND ALZHEIMER'S DISEASE.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Integrated Genetics Inc
1 Mountain Rd Framingham, MA 01701

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No