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TARGETING OF SELECTABLE MARKERS TO ANY GENETIC LOCUS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13978
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13978
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Integrated Genetics Inc
1 Mountain Rd Framingham, MA 01701
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: TARGETING OF SELECTABLE MARKERS TO ANY GENETIC LOCUS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

IT IS THE LONG-TERM GOAL OF THIS RESEARCH TO DEVELOP A METHOD OF ORDERLY SEGMENTING THE HUMAN GENOME INTO 1-50 MB FRAGMENTS. THIS OBJECTIVE CAN BE ACHIEVED BY STREAMLINING THE SCREENING PROCESS OF HYBRID CLONES GENERATED BY CHROMOSOME FRAGMENT GENE TRANSFER. THE MOST EXPEDITIOUS SCREENING PROCESS IS ONE THAT INCLUDES A DOMINANT SELECTABLE MARKER NEAR THE LOCUS OF INTEREST. HOMOLOGOUS RECOMBINATION WILL BE USED TO DIRECT A DOMINANT SELECTABLE MARKER TO A SPECIFIC CHROMOSOMAL LOCATION ALLOWING THE TARGETED LOCUS TO BECOME PHENOTYPICALLY SELECTABLE. CELLS CONTAINING A CORRECTLY TARGETED DOMINANT MARKER ARE AN IDEALDONOR SOURCE FOR GOSS-HARRIS HYBRIDS. THE TRANSGENOMES PRODUCED BY THIS APPROACH WILL BE VALUABLE REAGENTS FOR MOLECULAR AND MEDICAL GENETIC RESEARCH. THEY WILL ALLOW FOR A RAPID MEANS OF SATURATING A LOCUS WITH GENETIC MARKERS. IN ADDITION, THE RESULTING ENRICHMENT OF ATARGETED GENETIC DISEASE LOCUS WILL FACILITATE THE IDENTIFICATION OF THE RESPONSIBLE DEFECT. AT THE GENOME LEVEL, A SET OF TARGETING VECTORS, EACH DIRECTED TO CHROMOSOMAL LOCATIONS SEPARATED BY 10-20 MB, COULD GENERATE AN ORDERED SET OF TRANSGENOMES. THESE REAGENTS WOULD ENHANCE CURRENT AND FUTURE HUMAN GENOME MAPPING EFFORTS.

Principal Investigator:

Gregory M Landes
5088728400

Business Contact:

Small Business Information at Submission:

Integrated Genetics Inc
1 Mountain Rd Framingham, MA 01701

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No