USA flag logo/image

An Official Website of the United States Government

MECHANISMS\MICRONUCLEI INDUCTION IN MOUSE MARROW

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16572
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16572
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
INTEGRATED LABORATORY SYSTEMS, INC.
PO BOX 13501 RESEARCH TRIANGLE PARK, NC 27709-
View profile »
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: MECHANISMS\MICRONUCLEI INDUCTION IN MOUSE MARROW
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

DUE TO THE SPEED AND SIMPLICITY INVOLVED IN MICRONUCLEI (MN) IDENTIFICATION, MN ANALYSIS HAS BECOME INCREASINGLY USED AS A SENSITIVE, YET LESS EXPENSIVE AND TIME CONSUMING ALTERNATIVE TO CLASSICAL METAPHASE ANALYSIS. THE SCORING OF MN IS ONE OF THE FEW METHODS CURRENTLY AVAILABLE FOR THE DETECTION OF ANEUPLOID-RELATED PROCESSES. ALTHOUGH SEVERAL MN ASSAYS HAVE BEEN DEVELOPED, THE MOST WIDELY USED TEST SYSTEM INVOLVES THE SCORING OF MN IN BONE MARROW OR PERIPHERAL BLOOD POLYCHROMATIC ERYTHROCYTES (PCE) OF MICE. THE FACT THAT MN CAN ALSO ARISE THROUGH A MECHANISM INDEPENDENT OF DNA DAMAGE IS BOTH AN IMPORTANT ATTRIBUTE AND A CONFOUNDING FACTOR. THUS, THE DEVELOPMENT AND APPLICATION OF TECHNIQUES FOR IDENTIFYING THE MECHANISTIC ORIGIN OF MN ARE REQUIRED BEFORE THE INTERPRETATION OF MN DATA CAN EVER BECOME RELIABLE. THE DISCOVERY OF ANTIBODIES AGAINST CHROMOSOME KINETOCHORE REGIONS HAS MADE IT POSSIBLE TO IDENTIFY THE MECHANISTIC ORIGIN OF MN AND THIS METHOD HAS BEEN USED IN VITRO TO IDENTIFY MN WHICH ARISE FROM ABNORMAL CYTOKINESIS. THE PURPOSE OF THIS PROJECT IS TO DEVELOP AND APPLY THIS TECHNIQUE TO MN INDUCED IN MICE BY GENOTOXIC AGENTS.

Principal Investigator:

Tice, Raymond R
Principal Investigator
9195445857

Business Contact:

1 r43 es05381-01
Small Business Information at Submission:

Integrated Laboratory Systems
P O Box 13501 Res Triangle Park, NC 27709

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No