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Streamlined Template Preparation for Advanced Sequencing Methods

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44HG003946-02A1
Agency Tracking Number: HG003946
Amount: $1,471,850.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: PHS2007-2
Timeline
Solicitation Year: 2008
Award Year: 2008
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
INTELLIGENT BIO-SYSTEMS, INC. 34 Bear Hill Rd.
Waltham, MA 02451
United States
DUNS: 170777770
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 STEVEN GORDON
 (781) 466-8050
 SGORDON@INTELLIGENTBIOSYSTEMS.COM
Business Contact
Phone: (781) 466-8050
Email: sgordon@alum.mit.edu
Research Institution
N/A
Abstract

DESCRIPTION (provided by applicant): The advent of next-generation sequencing technologies is allowing researchers to perform studies and make discoveries which previously were not economically or technically feasible. For example, precise knowledge of th
e genomic sequence within neoplastic tissues can be an indicator for treatment (similar to the erbB-2 receptor gene, Her-2, for breast cancer). Although much research and development effort has gone into the core chemistries and instruments which actually
produce sequence data, few new techniques have been applied to the preparation of samples for high-throughput or use in the clinic. In particular, sequencing systems that use beads as both the solid support for amplification and to help localize samples on
a sequencing chip still require tedious processing by hand. As labs scale up to use the new sequencing technologies, they are very quickly realizing that the bottleneck in the operation is in the sample preparation. In this Phase II application, we propos
e to take the sample preparation steps far beyond the original Phase I scope of library construction. We propose to automate the entire sample preparation process, starting just after DNA fragmentation, through library construction and ending just prior to
attachment to a chip. The proposed system will be compatible with a number of next generation systems which are starting to enter the market.

* Information listed above is at the time of submission. *

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