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OF RNA

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4712
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4712
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
International Biotechnologies
Box 1565 275 Winchester Avenue New Haven, CT 06511
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: OF RNA
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

INTERNATIONAL TECHNOLOGIES HAS DEVISED A STRATEGY TO FACILITATE SEQUENCING METHODOLOGY BY USING PHAGE RNA POLYMERASES AND 3'-DEOXYNUCLEOTIDES AND MULTIPURPOSE VECTORS. THIS APPROACH IS EASIER AND FASTER THAN SANGER DIDEOXY SEQUENCING FOR SMALL FRAGMENTS, AS A FRAGMENT ONLY NEEDS TO BE CLONED IN ONE ORIENTATION TO SEQUENCE BOTH STRANDS. PROBLEMS ASSOCIATED WITH SECONDARY STRUCTURE IN SEQUENCING SHOULD BE ELIMINATED WITH THIS TECHNIQUE BECAUSE IT USES DOUBLE-STRANDED DNA AS TEMPLATE. THROUGH THE USE OFMULTIPURPOSE PIBI VECTORS, ONE COULD GO BACK AND FORTH BETWEEN SANGER DIDEOXY AND RNA POLYMERASE SEQUENCING DEPENDING UPON WHICH PROCEDURE GAVE OPTIMAL RESULTS.

Principal Investigator:

Roderic m k dale
PRINCIPAL INVESTIGATOR
2035623878

Business Contact:

Small Business Information at Submission:

International Biotechnologies
Box 1565 275 Winchester Avenue New Haven, CT 06511

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No