USA flag logo/image

An Official Website of the United States Government

Mass Spectrometry Assays for Biodefense Agents

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
75941
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
AI063651
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
INTRINSIC BIOPROBES, INC.
2155 E CONFERENCE DR STE 104 TEMPE, AZ 85284-2604
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2005
Title: Mass Spectrometry Assays for Biodefense Agents
Agency: HHS
Contract: 1R43AI063651-01
Award Amount: $295,015.00
 

Abstract:

DESCRIPTION (provided by applicant): The objective of this Phase I Research Proposal is to develop affinity-based mass spectrometry assay for detection of the NIAID High Priority Biodefense Agent Staphylococcal Enterotoxin B (SEB). The assay will utilize affinity capture in pipette-fitted microcolumns modified with anti-SEB antibody to selectively retrieve SEB from buffer, tap water, and milk samples. Detection will be achieved by measuring the molecular weight of the eluted SEB via MALDI-TOF mass spectrometry. The assay is unique as it offers dual specificity: the first comes from the use of a capturing antibody, and the second comes from the observation of a specific m/z value, characteristic of the analyzed toxin, in the resulting mass spectra. The use of polyclonal antibodies in combination with MS enables capture and detection of protein toxins and related variants in a single assay, and in the process significantly reduces the cost and time of analysis. In such, the proposed assay offers significant advantages over existing and commercially available sandwich-based SEB assays. Under the three specific aims under this proposal, we will demonstrate the reproducibility and sensitivity of the SEB assay, and develop and apply quantitative approaches for detection of SEB via the proposed affinity-based mass spectrometric assay. The successful completion of these Phase I Specific Aims will result in a fully-functional SEB-MSIA assay, and will lay the foundation for an experimental/developmental Phase II program that will focus on increasing the number of pathogen assays, developing multiplexed assays capable of detecting families and cohorts of toxins, integrating data evaluation methods into the assays, and describe the use of the assays and the accompanying instrumental platform in the laboratories that are part of the CDC's Laboratory Reference Network.

Principal Investigator:

Randall W. Nelson
4088041778
RNELSON@INTRINSICBIO.COM

Business Contact:

Dobrin Nedelkov
4808041778
DNEDELKOV@INTRINSICBIO.COM
Small Business Information at Submission:

Intrinsic Bioprobes, Inc.
Intrinsic Bioprobes, Inc. 625 S Smith Rd, Ste 22 Tempe, AZ 85281

EIN/Tax ID: 860842933
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No