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Quantitative Mass Spectrometric Immunoassays for Population Proteomics

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
94091
Program Year/Program:
2009 / SBIR
Agency Tracking Number:
RR025701
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
INTRINSIC BIOPROBES, INC.
2155 E CONFERENCE DR STE 104 TEMPE, AZ 85284-2604
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2009
Title: Quantitative Mass Spectrometric Immunoassays for Population Proteomics
Agency: HHS
Contract: 1R43RR025701-01A1
Award Amount: $197,319.00
 

Abstract:

DESCRIPTION (provided by applicant): The main objective of this Phase I project is to develop quantitative Mass Spectrometric Immunoassays for beta-2-microglobulin, cystatin C, transferrin, transthyretin, and retinol binding protein. These five proteins we re recently assayed via qualitative mass spectrometric immunoassays in 1,000 healthy cohorts, detecting (without providing concentration information) numerous protein variant. Because sandwich-based immunoassays such as ELISAs cannot specifically quantify the subtle protein modifications, in this project we propose to develop quantitative mass spectrometric immunoassays for these proteins and their isoforms. An internal reference standard will be carefully chosen, and a step-by-step standard curve quantitat ive mass spectrometric immunoassay development will be undertaken. The precision (intra-assay and inter-assay), linearity, sensitivity, and specificity of the quantitative mass spectrometric immunoassays will be determined. As a final validation step, the quantitative MSIA assays will be evaluated and benchmarked against existing ELISA assays. The quantitative mass spectrometric immunoassays proposed here will offer unique and straightforward means of quantifying protein variants, and when applied to diseas e cohorts, can provide valuable insight into the role of these variants in the onset of the disease, progression, and response to therapy. PUBLIC HEALTH RELEVANCE: The development of quantitative Mass Spectrometric Immunoassays for beta-2- microglobulin, c ystatin C, transferrin, transthyretin, and retinol binding protein will enable routine quantitative monitoring of these proteins and the changes in their modifications patterns that might be relevant to disease. With the new assays in hand, we will be bett er equipped to study the effects of these proteins' modifications in pathological processes and evaluate their potential as new indicators of the onset and progression of diseases, and response to therapy.

Principal Investigator:

Dobrin Nedelkov
4808041778
DNEDELKOV@INTRINSICBIO.COM

Business Contact:

Kemmons A. Tubbs
info@intrinsicbio.com
Small Business Information at Submission:

INTRINSIC BIOPROBES, INC.
INTRINSIC BIOPROBES, INC. 2155 E. Conference Dr. Suite 104 TEMPE, AZ 85284

EIN/Tax ID: 860842933
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No