DESCRIPTION: (Adapted from the Applicant's Abstact): LI-COR has combined solid-state optical technology with infrared fluorescent dye chemistry to detect and quantify DNA products with extremely high sensitivity. The applicant proposes to extend LI-COR's near-infrared (NIR) fluorescence technology… More

50942-98-I Open Path Carbon Dioxide Sensor--LI-COR, Inc., 4421 Superior Street, Lincoln, NE 68504-1327; (402) 467-3576 Dr. Jonathan M. Welles, Principal Investigator Dr. Gregory L. Biggs, Business Official DOE Grant No. DE-FG03-98ER82607 Amount: $75,000 Long term measurements of the… More

Not Available The 21st century Navy will need complete awareness of the surface and subsurface situations across a wide area of interest, in spite of the constraints of reduced budgets and manning levels, and the need for closer collaboration with other forces. To meet these new challenges, the… More

DESCRIPTION (provided by applicant): We are developing a new technology for single molecule DNA sequencing based on the incorporation of dNTPs labeled on the g-phosphate with fluorescent dyes. The label is cleaved from the nucleotide along with pyrophosphate when the nucleotide… More

DESCRIPTION (provided by applicant): We are developing a new technology for single molecule DNA sequencing based on the incorporation of dNTPs labeled on the g-phosphate with fluorescent dyes. The label is cleaved from the nucleotide along with pyrophosphate when the nucleotide… More

APPLICANT'S DESCRIPTION: DNA polymerases with altered substrate specificity are needed to support a new approach to single molecule DNA sequencing. Single molecule methods can be applied to DNA isolated directly from a subject organism, eliminating the need to clone, map and sort DNA fragments prior… More

We have been developing a system for real-time single molecule DNA sequencing. System components include novel "charge-switched" nucleotides, an adapted DNA polymerase, a method for isolating and handling single DNA molecules, a microfluidics controller and flowcell for sorting molecules by charge,… More

DESCRIPTION (provided by applicant): We have been developing a system for real-time single molecule DNA sequencing. System components include novel "charge-switched" nucleotides, an adapted DNA polymerase, a method for isolating and handling single DNA molecules, a microfluidics flowcell for sorting… More

78618S Robust and reliable trace-gas analyzers (e.g., for isotopic carbon, methane, nitroxide, ammonia, etc.), suitable for long-term measurements, are not commercially available. Such analyzers are needed to achieve a better understanding of the global budget of these trace gases and to quantify… More

DESCRIPTION (provided by applicant): We propose a new approach to single-molecule DMA sequencing that can be done in a simple microtiter plate fitted with two electrodes. Over two-hundred different single DMA molecules would be sequenced simultaneously in a single well (maximum 10 nt/s per… More

DESCRIPTION (provided by applicant): Fluorescent molecules that are in close proximity to metallic surfaces, including silver and gold island films, produce increased fluorescence intensity along with increased photostability and decreased fluorescent lifetimes. These metal island films have been… More

Robust and reliable trace-gas analyzers (e.g., for isotopic carbon, methane, nitroxide, ammonia, etc.), suitable for long-term measurements, are not commercially available. Such analyzers are needed to achieve a better understanding of the global budget of these trace gases and to quantify their… More

DESCRIPTION (provided by applicant): Fluorescent molecules that are positioned in close proximity to silver nanoparticles produce increased fluorescence intensity along with increased photostability and decreased lifetimes. These silver structures have bee n studied in great detail for their ability… More

A closed-path, methane and water vapor gas analyzer is required to support the widespread deployment that is needed to achieve a better understanding of the global methane budget and to quantify exchanges between the atmosphere and terrestrial biosphere. The analyzer must be robust, portable,… More

A closed-path methane and water vapor gas analyzer ¿ which is robust, portable, has low power consumption consistent with solar powered applications, uses room-temperature lasers, and is suitable for long-term and remote field measurements ¿ is not commercially available. Yet, such an analyzer is… More