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DNA polymerase for single molecule DNA sequencing

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
55658
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
2R44HG002292-02
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Li-cor Biosciences
4647 Superior Street Lincoln, NE 68504-1357
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: DNA polymerase for single molecule DNA sequencing
Agency: HHS
Contract: N/A
Award Amount: $0.00
 

Abstract:

DESCRIPTION (provided by applicant): We are developing a new technology for single molecule DNA sequencing based on the incorporation of dNTPs labeled on the g-phosphate with fluorescent dyes. The label is cleaved from the nucleotide along with pyrophosphate when the nucleotide is incorporated into DNA by a polymerase. The released label is sorted from intact g-dNTPs in a microfluidics system and is identified in real time by single molecule fluorescence detection optics. In this proposal, we develop a novel nucleotide chemistry to enable the sorting scheme and we use directed evolution methods to adapt polymerases for utilization of the g-dNTPs. Single molecule sequencing promises to reduce the cost of DNA sequencing by several orders of magnitude compared to current methods. PROPOSED COMMERCIAL APPLICATIONS: A genomic DNA sequencing technology that reduces cost and eliminates the need for cloning, physical mapping and electrophoresis would have great appeal to both the academic and industrial genomic research communities.

Principal Investigator:

John G. Williams

Business Contact:


4024673576
LYLEM@LICOR.COM
Small Business Information at Submission:

LI-COR, INC.
BOX 4425, 4421 SUPERIOR ST LINCOLN, NE 68504

EIN/Tax ID: 470537101
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No