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Electrokinetic Method For Single Molecule DNA Sequencing

Award Information

Department of Health and Human Services
Award ID:
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
Li-cor Biosciences
4647 Superior Street Lincoln, NE 68504-1357
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Phase 1
Fiscal Year: 2005
Title: Electrokinetic Method For Single Molecule DNA Sequencing
Agency: HHS
Contract: 1R43HG003610-01
Award Amount: $99,950.00


DESCRIPTION (provided by applicant): We propose a new approach to single-molecule DMA sequencing that can be done in a simple microtiter plate fitted with two electrodes. Over two-hundred different single DMA molecules would be sequenced simultaneously in a single well (maximum 10 nt/s per molecule) with read lengths 20 kb or more. Sequencing cost is potentially lower with this method compared to other long read approaches because of a high degree of multiplexing and because a simple microtiter plate substitutes for expensive micro or nanofabricated devices. While novel in concept, the proposed "electrokinetic" sequencing method would utilize modified polymerases and phosphate-labeled nucleotides from ongoing research on an alternative "charge-switch" method of single molecule sequencing. Ultrabright fluorescent nanoparticles and a prototype two-electrode sequencing chamber will be developed in Phase I. Particle transport kinetics will be characterized using the prototype chamber.

Principal Investigator:

John G. Williams

Business Contact:

Small Business Information at Submission:

Li-Cor Biosciences, Inc.
Li-Cor, Inc. 4308 Progressive Ave Lincoln, NE 68504

EIN/Tax ID: 470537101
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No