Metal Enhancement for Near-IR Fluorescence Based Membrane Assays
DESCRIPTION (provided by applicant): Fluorescent molecules that are positioned in close proximity to silver nanoparticles produce increased fluorescence intensity along with increased photostability and decreased lifetimes. These silver structures have bee
n studied in great detail for their ability to increase fluorescent intensities when deposited onto solid surfaces, such as glass or quartz. Little information exists, however, on the ability of silver nanostructures to enhance fluorophores deposited onto
porous membrane surfaces. The overall objective of this proposal is to use metal enhanced fluorescence to increase near- infrared fluorescence emissions on membrane substrates. Membrane based assays, such as western blots are routinely used in clinical set
tings to confirm serious diagnoses suggested by other screening tests, and in research settings to characterize and quantitate proteins of interest. Fluorescence based assays in the near-infrared offer the additional advantage of significantly lowering the
background compared to that generated in the visible spectrum, helping to lower the limit of detection. In the long term, this work should be applicable to most systems that utilize fluorescence in a manner that traps the fluorescent dyes on a membrane su
bstrate. Near-infrared metal enhanced fluorescence on membrane surfaces should provide a reliable and efficient procedure to increase fluorescent emissions and decrease the limit of detection, providing a tool that is compatible with current proteomic and
genomic applications. Future endeavors could use metal enhanced fluorescence to increase the detectability of membrane based DNA, protein, and antibody arrays on membranes. This project focuses on developing an enhancement reagent for Western and dot blot
analyses, which integrates the use of low background near-infrared LI-COR dyes with Metal Enhanced Fluorescence (MEF), to produce more sensitive membrane based assays capable of reducing the limit of detection by an order of magnitude. Advances in sensitiv
ity for these Western and dot blot assays will aid in the analysis of molecular, cellular, and inter-cellular processes, and the relationship of these processes with respect to one another.
Small Business Information at Submission:
LI-COR BIOSCIENCES, INC.
LI-COR, INC. 4308 PROGRESSIVE AVE LINCOLN, NE 68504
Number of Employees: