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Ubiquitin assisted production of multivalent vaccine in yeast

Award Information

Agency:
Department of Defense
Branch:
Office of the Secretary of Defense
Award ID:
61232
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
O012-0300
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LIFESENSORS, INC.
271 GREAT VALLEY PKY MALVERN, PA -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: Ubiquitin assisted production of multivalent vaccine in yeast
Agency / Branch: DOD / OSD
Contract: N00014-02-M-0273
Award Amount: $100,000.00
 

Abstract:

"The goal of this proposal is to demonstrate the feasibility to express in a short period of time a large number of malarial antigens by using ubiquitin fusion technology. We will design the yeast expression vectors for rapid cloning of a vast number ofmalarial genes. N-terminal of malarial antigens will be fused with C-terminus of ubiquitin to enhance quality and quantity of recombinant proteins in yeast. The following malarial candidate antigens will be included: multiepitope synthetic gene, FALVAC-1,AMA-1, CSP, EBA-175, LSA-1, MSP-1, PfHsp60, Pfs25, Pfs28 and SSP-2. These malarial antigens are components of the promising multivalent vaccine candidates. There is a consensus that an effective malaria vaccine will contain antigens or antigenicdeterminants from several stages. Thus, the methodology to express proteins quickly, and in large quantities will be an important advance. The success of this proposal will be proof of principle that new technique will allow cloning and expression of alarge number of antigens with improved quality and quantity in comparison with the existing methods. The presence of the multivalent antigen pool will serve as a vaccine candidate for immunological characterization in monkey model and further clinicaltesting. Anticipated improvement in protein expression and secretion will be of immediate use for protein production in general and for malaria antigens in particular. The success of Phase I will be c

Principal Investigator:

Hiep T. Tran
Asst. Director, R & D
6106448845
tran@lifesensors.com

Business Contact:

Hiep T. Tran
Asst Director, R & D
6106448845
tran@lifesensors.com
Small Business Information at Submission:

Lifesensors
271 Great Valley Parkway Malvern, PA 19355

EIN/Tax ID: 232855125
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No